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Mol Cell Biol

Title:		Mutations in a gene encoding the alpha subunit of a Saccharomyces cerevisiae G protein indicate a role in mating pheromone signaling
Author(s):		Jahng KY; Ferguson J; Reed SI;
Address:		"Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037"
Journal Title:		Mol Cell Biol
Year:		1988
Volume:		8
Issue:		6
Page Number:		2484 - 2493
DOI:		10.1128/mcb.8.6.2484-2493.1988
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"Mutations which allowed conjugation by Saccharomyces cerevisiae cells lacking a mating pheromone receptor gene were selected. One of the genes defined by such mutations was isolated from a yeast genomic library by complementation of a temperature-sensitive mutation and is identical to the gene GPA1 (also known as SCG1), recently shown to be highly homologous to genes encoding the alpha subunits of mammalian G proteins. Physiological analysis of temperature-sensitive gpa1 mutations suggests that the encoded G protein is involved in signaling in response to mating pheromones. Mutational disruption of G-protein activity causes cell-cycle arrest in G1, deposition of mating-specific cell surface agglutinins, and induction of pheromone-specific mRNAs, all of which are responses to pheromone in wild-type cells. In addition, mutants can conjugate without the benefit of mating pheromone or pheromone receptor. A model is presented where the activated G protein has a negative impact on a constitutive signal which normally keeps the pheromone response repressed"
Keywords:		"Agglutinins/metabolism Conjugation, Genetic Fungal Proteins/genetics/*physiology GTP-Binding Proteins/genetics/*physiology Mutation Phenotype Pheromones/*metabolism Saccharomyces cerevisiae/*physiology Transcription, Genetic *Transduction, Genetic;"
Notes:		"MedlineJahng, K Y Ferguson, J Reed, S I eng R01 GM38328/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1988/06/01 Mol Cell Biol. 1988 Jun; 8(6):2484-93. doi: 10.1128/mcb.8.6.2484-2493.1988"