| Title: | Molecular design of a eukaryotic messenger RNA and its chemical synthesis |
| Author(s): | Iwase R; Maeda M; Fujiwara T; Sekine M; Hata T; Miura K; |
| Address: | "Department of Life Chemistry, Tokyo Institute of Technology, Yokohama, Japan" |
| ISSN/ISBN: | 0305-1048 (Print) 1362-4962 (Electronic) 0305-1048 (Linking) |
| Abstract: | "A designed mRNA consisting of 42 ribonucleotides having the cap structure was synthesized. The capped leader sequence of the brome mosaic virus (BMV) mRNA 4, m7G5'pppGUAUUAAUA (F-1), was synthesized by the phosphotriester method and followed by the capping reaction. A 32-mer consisting of an initiation codon (AUG), the coding region corresponding to a bacterial pheromone cAD1 and two stop codons, was constructed by the 18-mer (F-2) and 14-mer (F-3), which were synthesized by the phosphoramidite method. 2'-,3'-O-Methoxymethylene-guanosine 5'-phosphate was condensed with F-3 using P1-2',3'-O-methoxymethyleneguanosine-5'-yl P2-adenosine-5'-yl pyrophosphate (9) with T4 RNA ligase. The chemically synthesized RNA fragments were ligated successively with T4 RNa ligase to afford the whole RNA molecule" |
| Keywords: | Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Codon/genetics Enterococcus faecalis/genetics Guanosine/*analogs & derivatives/chemistry/genetics Molecular Sequence Data Mosaic Viruses/genetics Oligopeptides/genetics Pheromones/genetics Phos; |
| Notes: | "MedlineIwase, R Maeda, M Fujiwara, T Sekine, M Hata, T Miura, K eng Research Support, Non-U.S. Gov't England 1992/04/11 Nucleic Acids Res. 1992 Apr 11; 20(7):1643-8. doi: 10.1093/nar/20.7.1643" |
