| Title: | Molecular heterogeneity in major urinary proteins of Mus musculus subspecies: potential candidates involved in speciation |
| Author(s): | Hurst JL; Beynon RJ; Armstrong SD; Davidson AJ; Roberts SA; Gomez-Baena G; Smadja CM; Ganem G; |
| Address: | "Mammalian Behaviour and Evolution Group, Institute of Integrative Biology, University of Liverpool, Leahurst Campus, Neston, CH64 7TE, UK. Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK. Institut des Sciences de l'Evolution de Montpellier UMR5554 (UM, CNRS, IRD, EPHE), Universite de Montpellier, CC 065, 34095 Montpellier Cedex 05, France" |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "When hybridisation carries a cost, natural selection is predicted to favour evolution of traits that allow assortative mating (reinforcement). Incipient speciation between the two European house mouse subspecies, Mus musculus domesticus and M.m.musculus, sharing a hybrid zone, provides an opportunity to understand evolution of assortative mating at a molecular level. Mouse urine odours allow subspecific mate discrimination, with assortative preferences evident in the hybrid zone but not in allopatry. Here we assess the potential of MUPs (major urinary proteins) as candidates for signal divergence by comparing MUP expression in urine samples from the Danish hybrid zone border (contact) and from allopatric populations. Mass spectrometric characterisation identified novel MUPs in both subspecies involving mostly new combinations of amino acid changes previously observed in M.m.domesticus. The subspecies expressed distinct MUP signatures, with most MUPs expressed by only one subspecies. Expression of at least eight MUPs showed significant subspecies divergence both in allopatry and contact zone. Another seven MUPs showed divergence in expression between the subspecies only in the contact zone, consistent with divergence by reinforcement. These proteins are candidates for the semiochemical barrier to hybridisation, providing an opportunity to characterise the nature and evolution of a putative species recognition signal" |
| Keywords: | "Animals Europe Evolution, Molecular Female *Genetic Heterogeneity *Genetic Variation Geography Male Mice Proteins/*genetics/metabolism Proteome Proteomics/methods Selection, Genetic Species Specificity;" |
| Notes: | "MedlineHurst, Jane L Beynon, Robert J Armstrong, Stuart D Davidson, Amanda J Roberts, Sarah A Gomez-Baena, Guadalupe Smadja, Carole M Ganem, Guila eng BBC503897/Biotechnology and Biological Sciences Research Council/United Kingdom BB/J002631/1/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2017/03/25 Sci Rep. 2017 Mar 24; 7:44992. doi: 10.1038/srep44992" |
