| Title: | Inhibition of membrane Ca2(+)-ATPase of Saccharomyces cerevisiae by mating pheromone alpha-factor in vitro |
| Author(s): | Hiraga K; Tahara H; Taguchi N; Tsuchiya E; Fukui S; Miyakawa T; |
| Address: | "Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan" |
| ISSN/ISBN: | 0022-1287 (Print) 0022-1287 (Linking) |
| Abstract: | "Plasma membrane Ca2(+)-ATPase of Saccharomyces cerevisiae was solubilized and partially purified by calmodulin-affinity chromatography. The activity of Ca2(+)-ATPase isolated from MATa cells was inhibited by physiological concentrations of the mating pheromone alpha-factor in a dose-dependent manner. The enzyme prepared from a receptor-deficient sterile mutant cells (delta ste-2) was similarly inhibited by alpha-factor, but the enzyme from MAT alpha cells was resistant to the mating pheromone. We suggest that the inhibition may be involved in the alpha-factor-induced increase of Ca2+ uptake reaction of MATa cells" |
| Keywords: | "Bacterial Proteins/pharmacology Calcium-Transporting ATPases/*antagonists & inhibitors Cell Membrane/drug effects/enzymology Chromatography, Affinity In Vitro Techniques Mating Factor Mutation Peptides/*pharmacology Pheromones/*pharmacology Saccharomyces;" |
| Notes: | "MedlineHiraga, K Tahara, H Taguchi, N Tsuchiya, E Fukui, S Miyakawa, T eng England 1991/01/01 J Gen Microbiol. 1991 Jan; 137(1):1-4. doi: 10.1099/00221287-137-1-1" |
