| Title: | Determination of tuberculosis-related volatile organic biomarker methyl nicotinate in vapor using fluorescent assay based on quantum dots and cobalt-containing porphyrin nanosheets |
| Author(s): | He Q; Cai S; Wu J; Hu O; Liang L; Chen Z; |
| Address: | "School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, People's Republic of China. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, People's Republic of China. chenzg@mail.sysu.edu.cn" |
| DOI: | 10.1007/s00604-022-05212-w |
| ISSN/ISBN: | 1436-5073 (Electronic) 0026-3672 (Linking) |
| Abstract: | "Methyl nicotinate (MN) is a representative and typical volatile organic marker of Mycobacterium tuberculosis, and the specific detection of MN in human breath facilitates non-invasive, rapid, and accurate epidemic screening of tuberculosis infection. Herein, we constructed a fluorescent assay consisted of CdTe quantum dots (QD) and cobalt-metalized tetrakis(4-carboxyphenyl) porphyrin (CoTCPP) nanosheets to determine methyl nicotinate (MN) in vapor samples. Red-emission QD (lambda(ex)=370 nm, lambda(em)=658 nm) acts as signal switches whose fluorescence signals can be effectively quenched by CoTCPP nanosheets but restored in the presence of MN. The strategy relied on the distinct binding affinity of cobalt ion and MN. MN restored the fluorescence of QD quenched by CoTCPP in a concentration-dependent manner, which exhibited a well-linear relationship in the range 1-100 muM, and a limit of detection of 0.59 muM. The proposed platform showed sensitivity and selectivity to detect MN in vapor samples with satisfactory RSD below 3.33%. The method is cheap, simple, and relatively rapid (detected within 4 min), which suggests a potential in tuberculosis diagnosis in resource- and professional-lacked areas" |
| Keywords: | "Biomarkers/analysis Cadmium Compounds/*chemistry Humans Mycobacterium tuberculosis/*chemistry Nanoparticles/*chemistry Nicotinic Acids/*analysis Porphyrins/*chemistry Quantum Dots/*chemistry Spectrometry, Fluorescence Tellurium/*chemistry Volatilization F;" |
| Notes: | "MedlineHe, Qidi Cai, Shuangshuang Wu, Jinghao Hu, Ou Liang, Lushan Chen, Zuanguang eng No. 202002020084/Science and Technology Planning Project of Guangzhou/ No. 21675177/National Natural Science Foundation of China/ No. 20191014/State Administration of Traditional Chinese Medicine of the People's Republic of China/ Research Support, Non-U.S. Gov't Austria 2022/02/17 Mikrochim Acta. 2022 Feb 16; 189(3):108. doi: 10.1007/s00604-022-05212-w" |
