| Title: | Monitoring of Campylobacter jejuni in a chicken infection model by measuring specific volatile organic compounds and by qPCR |
| Author(s): | Hankel J; Gibson T; Skov J; Andersen KB; Dargatz M; Kappel A; Thiemann F; Curtis B; Chuppava B; Visscher C; |
| Address: | "Institute for Animal Nutrition, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173, Hannover, Germany. RoboScientific Ltd, Espace North, 181 Wisbech Road, Littleport, CB6 1RA, Cambridgeshire, UK. AeroCollect A/S, Park Alle 345, 2605, Brondby, Denmark. Evonik Operations GmbH, Nutrition & Care, Rodenbacher Chaussee 4, 63457, Hanau-Wolfgang, Germany. Institute for Animal Nutrition, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173, Hannover, Germany. bussarakam.chuppava@tiho-hannover.de" |
| DOI: | 10.1038/s41598-022-15863-7 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Campylobacter is one of the leading bacterial foodborne pathogens worldwide. Poultry is the host species with this pathogen with the highest clinical impact. Flocks become colonised with Campylobacter, which leads to contamination of product entering the food-chain. Rapid and reliable Campylobacter detection methods could support controls to minimize the risks of contamination within the food-chain, which would easier enable the implementation of a logistical slaughter schedule or other control options. The present study evaluates current and emerging C. jejuni detection technologies on air samples in a unique study set-up of pre-defined C. jejuni prevalences. Both non-invasive detection technologies on air samples by subsequent measuring of volatile organic compounds (VOCs) or by qPCR detected the C. jejuni presence and could additionally distinguish between the number of present C. jejuni-positive birds in the study set-up. Nevertheless, electrostatic air samplers diagnosed fewer birds as C. jejuni-positive compared to the cultivation-based method. By measuring the VOCs, it was possible to detect the presence of two positive birds in the room. This apparent high sensitivity still needs to be verified in field studies. Techniques, such as these promising methods, that can facilitate C. jejuni surveillance in poultry flocks are desirable to reduce the risk of infection for humans" |
| Keywords: | Animals *Campylobacter *Campylobacter Infections/diagnosis/microbiology/veterinary *Campylobacter jejuni/genetics Chickens/microbiology Humans Poultry *Poultry Diseases/diagnosis/microbiology *Volatile Organic Compounds; |
| Notes: | "MedlineHankel, Julia Gibson, Timothy Skov, Julia Andersen, Karsten Brandt Dargatz, Michelle Kappel, Andreas Thiemann, Frank Curtis, Ben Chuppava, Bussarakam Visscher, Christian eng Research Support, Non-U.S. Gov't England 2022/07/14 Sci Rep. 2022 Jul 11; 12(1):11725. doi: 10.1038/s41598-022-15863-7" |
