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Mar Drugs

Title:		Bioprospecting of Less-Polar Constituents from Endemic Brown Macroalga Fucus virsoides J. Agardh from the Adriatic Sea and Targeted Antioxidant Effects In Vitro and In Vivo (Zebrafish Model)
Author(s):		Jerkovic I; Cikos AM; Babic S; Cizmek L; Bojanic K; Aladic K; Ul'yanovskii NV; Kosyakov DS; Lebedev AT; Coz-Rakovac R; Trebse P; Jokic S;
Address:		"Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Rudera Boskovica 35, 21000 Split, Croatia. Department of Process Engineering, Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhaca 18, 31000 Osijek, Croatia. Laboratory for Aquaculture Biotechnology, Division of Materials Chemistry, Ruder Boskovic Institute, Bijenicka cesta 54, 10000 Zagreb, Croatia. Laboratory of Environmental Analytical Chemistry, Core Facility Center 'Arktika', Northern (Arctic) Federal University, Naberezhnaya Severnoy Dviny 17, 163002 Arkhangelsk, Russia. Department of Organic Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia. Faculty of Health Sciences, University of Ljubljana, Zdravstvena pot 5, 1000 Ljubljana, Slovenia"
Journal Title:		Mar Drugs
Year:		2021
Volume:		20210422
Issue:		5
Page Number:		-
DOI:		10.3390/md19050235
ISSN/ISBN:		1660-3397 (Electronic) 1660-3397 (Linking)
Abstract:		"The endemic brown macroalga Fucus virsoides J. Agardh from the Adriatic Sea was in the focus of the present research. The volatiles of fresh (FrFv) and air-dried (DrFv) samples of F. virsoides obtained by headspace solid-phase microextraction (HS-SPME) and hydrodistillation (HD) were analyzed by gas chromatography equipped with flame ionization detector and mass spectrometry (GC-FID/MS). The major HS-FrFv compound was pentadecane (61.90-71.55%) followed by pentadec-1-ene (11.00-7.98%). In HS-DrFv, pentadec-1-ene was not present, and few lower aliphatic compounds appeared, as well as benzaldehyde and benzyl alcohol. In HD-FrFv, particularly abundant were alkenes (such as pentadec-1-ene (19.32%), or (E)-pentadec-7-ene (8.35%)). In HD-DrFv, more oxidation products were present (e.g., carbonyl compounds such as tridecanal (18.51%)). The fatty acids profile of freeze-dried sample (FdFv) after conversion to methyl esters was determined by GC-FID, and oleic acid was dominant (42.28%), followed by arachidonic acid (15.00%). High-performance liquid chromatography-high-resolution mass spectrometry with electrospray ionization (HPLC-ESI-HRMS) was used for the screening of less polar fractions (F3 and F4) of F. virsoides. Mono- and diglycerides of stearic, palmitic, oleic, and arachidonic acids were found. Terpenoids and steroids comprised the compounds C(20)H(30(32))O(2) and C(29)H(48)O((2)). Among carotenoids, fucoxanthin was identified. Chlorophyll derivatives were also found (C(55)H(74(72))N(4)O((5-7))), dominated by pheophytin a. The antioxidant activity of the fractions was investigated by in vitro assays (oxygen radical absorbance capacity (ORAC), reduction of radical cation (ABTS*(+)), 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay, and ferric reducing antioxidant power (FRAP)) and by in vivo zebrafish model (along with fish embryotoxicity). In vitro experiments proved good radical scavenging abilities of F3 and F4 fractions, which were additionally supported by the protective effect against hydrogen peroxide-induced oxidative stress in zebrafish embryos"
Keywords:		Animals Anti-Bacterial Agents/isolation & purification/pharmacology Antifungal Agents/isolation & purification/pharmacology Antioxidants/isolation & purification/*pharmacology *Bioprospecting *Drug Discovery Fucus/*metabolism Microbial Sensitivity Tests O;
Notes:		"MedlineJerkovic, Igor Cikos, Ana-Marija Babic, Sanja Cizmek, Lara Bojanic, Krunoslav Aladic, Krunoslav Ul'yanovskii, Nikolay V Kosyakov, Dmitry S Lebedev, Albert T Coz-Rakovac, Rozelindra Trebse, Polonca Jokic, Stela eng KK.01.1.1.01/the Croatian Government and the European Union through the European Regional Development Fund - the Competitiveness and Cohesion Operational Pro-gramme/ Comparative Study Switzerland 2021/05/01 Mar Drugs. 2021 Apr 22; 19(5):235. doi: 10.3390/md19050235"