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J Bacteriol

Title:		"ccfA, the genetic determinant for the cCF10 peptide pheromone in Enterococcus faecalis OG1RF"
Author(s):		Antiporta MH; Dunny GM;
Address:		"Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455-0312, USA"
Journal Title:		J Bacteriol
Year:		2002
Volume:		184
Issue:		4
Page Number:		1155 - 1162
DOI:		10.1128/jb.184.4.1155-1162.2002
ISSN/ISBN:		0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking)
Abstract:		"The nosocomial pathogen Enterococcus faecalis has a unique pheromone-inducible conjugative mating system. Conjugative transfer of the E. faecalis plasmid pCF10 is specifically induced by the cCF10 peptide pheromone (LVTLVFV). Genomic sequence information has recently allowed the identification of putative structural genes coding for the various enterococcal pheromones (D. B. Clewell et al., Mol. Microbiol. 35:246-247, 2000). The cCF10 pheromone sequence LVTLVFV was found within an open reading frame designated ccfA, encoding a putative lipoprotein precursor. Several other pheromone sequences were found in similar locations within other predicted lipoproteins. CcfA shows significant sequence relatedness to the Escherichia coli protein YidC, an inner membrane protein translocase, as well as to a large number of homologs identified in gram-positive and in gram-negative bacteria. Analysis of the deduced CcfA amino acid sequence suggested that mature cCF10 peptide could be formed from the proteolytic degradation of its signal peptide. Expression of the cloned ccfA gene with an inducible expression vector dramatically increased cCF10 production by E. faecalis and also resulted in cCF10 production by Lactococcus lactis, a non-pheromone producer. Site-directed mutagenesis of the ccfA sequence encoding the cCF10 peptide confirmed that ccfA was a functional genetic determinant for cCF10"
Keywords:		"Amino Acid Sequence Bacterial Proteins/*genetics Base Sequence Cloning, Molecular Enterococcus faecalis/*genetics/metabolism Gene Expression Genes, Bacterial Lipoproteins/*genetics Molecular Sequence Data Oligopeptides/biosynthesis/*genetics *Open Reading;"
Notes:		"MedlineAntiporta, Michelle H Dunny, Gary M eng R01 GM049530/GM/NIGMS NIH HHS/ GM49350/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2002/01/25 J Bacteriol. 2002 Feb; 184(4):1155-62. doi: 10.1128/jb.184.4.1155-1162.2002"