| Title: | Acute inhalation toxicity study of n-heneicosane and its combination with diflubenzuron: An attracticide of Aedes aegypti |
| Author(s): | Gautam A; Singh D; Shrivastava P; Vijayaraghavan R; |
| Address: | "1 Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi, India. 2 Department of Pharmacology, Khalsa College of Pharmacy, Amritsar, India. 3 QuentilesIMS, Bangalore, India. 4 Saveetha University, Chennai, India" |
| ISSN/ISBN: | 1477-0393 (Electronic) 0748-2337 (Linking) |
| Abstract: | "OBJECTIVE: Combination of an oviposition pheromone and an insect growth regulator for the control of vectors is an effective approach. There is a need for toxicological evaluation before its introduction. The present study evaluates the acute inhalation toxicity of n-heneicosane and its combination with diflubenzuron in a head-only inhalation exposure chamber made of glass. MATERIALS AND METHODS: A head-only inhalation exposure chamber made of glass (volume: 3.5 l) was used for exposing four rats at a time. A glass nebulizer was used for aerosolization of n-heneicosane and its combination with diflubenzuron (1:10 w/w). Nebulization pressure was 10 and 15 psi and the air flow of exposure the chamber was adjusted to 30 lpm. Male Wistar rats were acclimatized in whole body plethysmographs that were connected to volumetric flow pressure transducers by silicon tubes. The transducers were connected to an amplifier and a digitized response was recorded through an oscillograph and personal computers. Respiratory variables were recorded online. After inhalation exposure, various other parameters like survival, body weight, organ body weight index and biochemical changes were recorded for analysis. RESULTS AND DISCUSSION: Particle size determination proved that the aerosol particles were within the respirable range. LC(50) of n-heneicosane and its combination with diflubenzuron was found to be more than 5 g/m(3). There were minimal changes observed during exposure to n-heneicosane and also its combination with diflubenzuron on the respiratory variables. The changes were not consistent with the dose. CONCLUSION: n-Heneicosane and its combination with diflubenzuron showed low mammalian toxicity" |
| Keywords: | Aedes Alkanes/administration & dosage/*toxicity Animals Heart/drug effects Inhalation Exposure Insect Repellents/administration & dosage/*toxicity Kidney/drug effects Liver/drug effects Lung/drug effects Male Nebulizers and Vaporizers Organ Size/drug effe; |
| Notes: | "MedlineGautam, Anshoo Singh, Deeksha Shrivastava, Peeyush Vijayaraghavan, R eng England 2018/07/24 Toxicol Ind Health. 2018 Oct; 34(10):703-713. doi: 10.1177/0748233718774987. Epub 2018 Jul 22" |
