Title: | Analysis of a transfer region from the staphylococcal conjugative plasmid pSK41 |
Author(s): | Firth N; Ridgway KP; Byrne ME; Fink PD; Johnson L; Paulsen IT; Skurray RA; |
Address: | "School of Biological Sciences, University of Sydney, NSW, Australia" |
DOI: | 10.1016/0378-1119(93)90442-6 |
ISSN/ISBN: | 0378-1119 (Print) 0378-1119 (Linking) |
Abstract: | "The nucleotide sequence of a 14.4-kb region (tra) associated with DNA transfer of the staphylococcal conjugative plasmid, pSK41, has been determined. Analysis of the sequence revealed the presence of 15 genes potentially involved in the conjugative process. Polypeptide products likely to correspond to ten of these genes have been identified, of which one was found to be a lipoprotein. Comparison of the deduced tra products to the protein databases revealed several interesting similarities, one of which suggests an evolutionary link between this Gram+ bacterial conjugation system and DNA transfer systems of Gram- bacteria, such as Escherichia coli and Agrobacterium tumefaciens. The nt sequence also provided an insight into the transcriptional organisation and regulation of the region" |
Keywords: | "Amino Acid Sequence Bacterial Proteins/*genetics Base Sequence Biological Evolution Cloning, Molecular Conjugation, Genetic/genetics DNA, Bacterial Lipoproteins/genetics Molecular Sequence Data Pheromones/genetics Plasmids/*genetics Protein Sorting Signal;" |
Notes: | "MedlineFirth, N Ridgway, K P Byrne, M E Fink, P D Johnson, L Paulsen, I T Skurray, R A eng Research Support, Non-U.S. Gov't Netherlands 1993/12/22 Gene. 1993 Dec 22; 136(1-2):13-25. doi: 10.1016/0378-1119(93)90442-6" |