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Proc Natl Acad Sci U S A

Title:		Control of yeast alpha-specific genes: evidence for two blocks to expression in MATa/MAT alpha diploids
Author(s):		Ammerer G; Sprague GF; Bender A;
Address:
Journal Title:		Proc Natl Acad Sci U S A
Year:		1985
Volume:		82
Issue:		17
Page Number:		5855 - 5859
DOI:		10.1073/pnas.82.17.5855
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"In yeast alpha cells, the product encoded by the MAT alpha 1 gene of the mating-type locus is required for transcription of at least two genes, STE3 and MF alpha 1. To learn whether the lack of the MAT alpha 1 product in a and a/alpha cells is sufficient to explain the failure to express STE3 and MF alpha 1 in these cells, we have provided MAT alpha 1 product via a hybrid gene that circumvents the normal regulation of the MAT alpha 1 gene. We find by RNA blot analysis that provision of MAT alpha 1 protein permits production of STE3 and MF alpha 1 mRNA in a cells but not in a/alpha cells. These data suggest the existence of an additional regulatory mechanism that prevents expression of alpha-specific genes in a/alpha cells, even when MAT alpha 1 product is present. This regulatory mechanism appears to control expression of STE3 and MF alpha 1 at the transcriptional level rather than at the posttranscriptional level, because we show that MF alpha 1 mRNA supplied from a constitutive promoter is translated and processed in a/alpha cells to yield functional alpha-factor pheromone. This result shows further that a/alpha cells possess all the machinery necessary for pheromone maturation, even though these cells do not normally carry out these reactions"
Keywords:		"Animals DNA, Recombinant Diploidy Fungal Proteins/*genetics Gene Expression Regulation *Genes, Fungal *Genes, Mating Type, Fungal Genes, Regulator Mating Factor Peptides/genetics Pheromones/*genetics Promoter Regions, Genetic RNA, Fungal/genetics RNA, Mes;"
Notes:		"MedlineAmmerer, G Sprague, G F Jr Bender, A eng GM00715/GM/NIGMS NIH HHS/ GM11895/GM/NIGMS NIH HHS/ GM30027/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1985/09/01 Proc Natl Acad Sci U S A. 1985 Sep; 82(17):5855-9. doi: 10.1073/pnas.82.17.5855"