| Title: | Effects of commercial aldehydes from green leaf volatiles (green odour) on rumen microbial population and fermentation profile in an artificial rumen (Rusitec) |
| Author(s): | Demirtas A; Ozturk H; Sudagidan M; Keyvan E; Yavuz O; Gulay OY; Musa SAA; |
| Address: | "Department of Physiology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, 15030, Burdur, Turkey. Electronic address: ahu-demirtas@hotmail.com. Department of Physiology, Faculty of Veterinary Medicine, Ankara University, 06110, Diskapi, Ankara, Turkey. Electronic address: ozturkh@veterinary.ankara.edu.tr. KIT-ARGEM R&D Center and SARGEM R&D Center, Konya Food and Agriculture University, 42080, Meram, Konya, Turkey. Electronic address: msudagidan@gmail.com. Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, 15030, Burdur, Turkey. Electronic address: erhankeyvan@mehmetakif.edu.tr. Scientific and Technology Application and Research Center, Mehmet Akif Ersoy University, 15030, Burdur, Turkey. Electronic address: oyavuz@mehmetakif.edu.tr. Department of Physiology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, 15030, Burdur, Turkey. Electronic address: oygulay@mehmetakif.edu.tr. Department of Physiology, Faculty of Veterinary Medicine, Ankara University, 06110, Diskapi, Ankara, Turkey. Electronic address: saadbutana@gmail.com" |
| DOI: | 10.1016/j.anaerobe.2018.11.001 |
| ISSN/ISBN: | 1095-8274 (Electronic) 1075-9964 (Linking) |
| Abstract: | "The effects of plant metabolites on rumen metabolism vary greatly depending on their antimicrobial spectrum and applied doses. In this study, the minimum inhibitory concentrations (MICs) of commercial aldehydes, trans-2-hexenal (T2H), cis-3-hexenal (C3H), trans-2-nonenal (T2N), and trans-2-decenal (T2D) from green leaf volatiles, were tested on rumen bacteria. These compounds were found more effective on Gram-positive rumen bacteria than the Gram-negatives, and C3H was the most effective compound. Then, for 14 days, the in vitro effects of C3H compared with monensin (5?ª+mg/day) on the rumen microbial population and ruminal fermentation at 187.5, 375 and 750?ª+mg/day doses were tested based on the MIC value (500?ª+mug/mL) by using the rumen simulation technique (Rusitec). Supplementation with C3H at 375?ª+mg/day increased the cell numbers of Butyrivibrio fibrisolvens significantly. The addition of C3H at 375 and 750?ª+mg/day doses also increased Streptecoccus bovis cell counts. The use of monensin did not affect the cell numbers of these bacteria. On the other hand, C3H did not change the counts of total bacteria, methanogens, or hyper-ammonia-producing (HAP) bacteria like monensin. The numbers of Ruminococcus albus and Ruminococcus flavefaciens were also stable in the presence of C3H but decreased significantly with the addition of monensin (P?ª+ |
| Keywords: | "Aldehydes/isolation & purification/*metabolism Animals Bacterial Load Biota/*drug effects Fermentation/drug effects Gram-Negative Bacteria/drug effects Gram-Positive Bacteria/drug effects Microbial Sensitivity Tests Models, Biological Plant Leaves/*chemis;" |
| Notes: | "MedlineDemirtas, Ahu Ozturk, Hakan Sudagidan, Mert Keyvan, Erhan Yavuz, Orhan Gulay, Ozlem Yildiz Musa, Saad Ahmed Adam eng England 2018/11/06 Anaerobe. 2019 Feb; 55:83-92. doi: 10.1016/j.anaerobe.2018.11.001. Epub 2018 Nov 3" |
