| Title: | Microbial volatile organic compounds 2-heptanol and acetoin control Fusarium crown and root rot of tomato |
| Author(s): | Du J; Ji Y; Li Y; Liu B; Yu Y; Chen D; Li Z; Zhao T; Xu X; Chang Q; Li Z; Li P; Jiang Y; Chen Y; Lu C; Wei L; Wang C; Li Y; Yin Z; Kong L; Ding X; |
| Address: | "State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of plant protection, Shandong Agricultural University, Tai'an, Shandong, P.R. China. Shandong Pengbo Biotechnology Co., LTD, Tai'an, Shandong, P.R. China. Yantai Academy of Agricultural Sciences, Yantai, Shandong, P.R. China. College of Life Sciences, Zaozhuang University, Zaozhuang, Shandong, P.R. China" |
| ISSN/ISBN: | 1097-4652 (Electronic) 0021-9541 (Linking) |
| Abstract: | "Some microbial volatile organic compounds (mVOCs) can act as antagonistic weapons against plant pathogens, but little information is available on the contribution of individual mVOC to biocontrol and how they interact with plant pathogens. In this study, the Bacillus subtilis strain N-18 isolated from the rhizosphere of healthy plants grown in areas where Fusarium crown and root rot (FCRR) of tomato occurs could reduce the 30% of the incidence of FCRR. Moreover, the volatile organic compounds (VOCs) produced by N-18 had inhibitory effects on Fusarium oxysporum f. sp. radicis-lycopersici (FORL). The identification of VOCs of N-18 was analyzed by the solid-phase microextraction coupled to gas chromatography-mass spectrometry. Meanwhile, we conducted sensitivity tests with these potential active ingredients and found that the volatile substances acetoin and 2-heptanol can reduce the 41.33% and 35% of the incidence of FCRR in tomato plants. In addition, the potential target protein of acetoin, found in the cheminformatics and bioinformatics database, was F. oxysporum of hypothetical protein AU210_012600 (FUSOX). Molecular docking results further predicted that acetoin interacts with FUSOX protein. These results reveal the VOCs of N-18 and their active ingredients in response to FORL and provide a basis for further research on regulating and controlling FCRR" |
| Keywords: | 2-heptanol Fusarium oxysporum f.sp.radicis-lycopersici acetoin biological control of diseases cheminformatics and bioinformatics target protein; |
| Notes: | "PublisherDu, Jianfeng Ji, Yatai Li, Yue Liu, Baoyou Yu, Yiming Chen, Dayin Li, Zhiwei Zhao, Tianfeng Xu, Xinning Chang, Qingle Li, Zimeng Li, Pengan Jiang, Yanke Chen, Yudong Lu, Chongchong Wei, Lansu Wang, Cunchen Li, Yang Yin, Ziyi Kong, Lingguang Ding, Xinhua eng 32072500/National Natural Science Foundation of China/ 31872925/National Natural Science Foundation of China/ 31801845/National Natural Science Foundation of China/ 2019KJF023/Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province/ 2020CXGC010803/Shandong Province Key Research and Development Plan/ 2021TZXD007-04-4/Shandong Province Key Research and Development Plan/ ZR2020QC130/Shandong Natural Science Foundation Youth Project/ 2022/10/03 J Cell Physiol. 2022 Oct 2. doi: 10.1002/jcp.30889" |
