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Rapid Commun Mass Spectrom

Title:		"Influence of the eluent composition on the ionization efficiency for morphine of pneumatically assisted electrospray, atmospheric-pressure chemical ionization and sonic spray"
Author(s):		Dams R; Benijts T; Gunther W; Lambert W; De Leenheer A;
Address:		"Laboratorium voor Toxicologie, Universiteit Gent, Harelbekestraat 72, B-9000 Gent, Belgium"
Journal Title:		Rapid Commun Mass Spectrom
Year:		2002
Volume:		16
Issue:		11
Page Number:		1072 - 1077
DOI:		10.1002/rcm.683
ISSN/ISBN:		0951-4198 (Print) 0951-4198 (Linking)
Abstract:		"A comparative study of three atmospheric-pressure ionization (API) sources for liquid chromatography/mass spectrometry (LC/MS), namely pneumatically assisted electrospray or ionspray (IS), atmospheric-pressure chemical ionization (APCI), and sonic spray (SS), with respect to the influence of the eluent composition on the ionization of morphine, is presented. The effect of organic modifiers, volatile acids, and buffer systems (with and without pH adjustment) in the LC mobile phase on the ionization efficiency of each interface is described. We conclude that for all three ion sources, the composition of the liquid phase had a serious impact on the ionization of the target compound. For IS and SS, very similar behavior towards the LC eluent was observed. In both cases, an increase in organic modifier resulted in an increase in ionization, while an increasing amount of volatile acid or buffer caused signal suppression. APCI, on the other hand, proved to respond completely differently towards the changes in the eluent. Again, an increased ionization was observed with an increase in organic modifier content but this time also in the presence of mobile phase additives like acids or buffers. Finally, we concluded that APCI proved to be the preferred ion source for the test compound because of its robust character and its direct applicability in traditional LC analysis"
Keywords:		"Chromatography, Liquid/methods Morphine/*analysis Spectrometry, Mass, Electrospray Ionization/instrumentation/*methods;"
Notes:		"MedlineDams, Riet Benijts, Tom Gunther, Wolfgang Lambert, Willy De Leenheer, Andre eng Comparative Study Research Support, Non-U.S. Gov't England 2002/05/07 Rapid Commun Mass Spectrom. 2002; 16(11):1072-7. doi: 10.1002/rcm.683"