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Peptides

Title:		Aplysia seductin is a water-borne protein pheromone that acts in concert with attractin to stimulate mate attraction
Author(s):		Cummins SF; Nichols AE; Warso CJ; Nagle GT;
Address:		"Department of Neuroscience and Cell Biology, Marine Biomedical Institute, University of Texas Medical Branch, 11th Mechanic Street Rm 2.138, Galveston, TX 77555-1069, USA"
Journal Title:		Peptides
Year:		2005
Volume:		26
Issue:		3
Page Number:		351 - 359
DOI:		10.1016/j.peptides.2004.10.024
ISSN/ISBN:		0196-9781 (Print) 0196-9781 (Linking)
Abstract:		"Mate attraction in Aplysia involves the long-distance water-borne protein pheromones attractin, enticin, and temptin which are released during egg-laying. Other water-borne pheromones are predicted to act in concert with attractin, enticin, and temptin, but their identities were unknown. We recently identified a highly expressed Aplysia californica albumen gland gene (Alb-23) that encoded a novel protein by differential library screening of an albumen gland cDNA library. To determine whether Alb-23 ('seductin') was a water-borne pheromone, we employed Western blot analysis, purification and expression of albumen gland proteins, immunolocalization studies, pheromone secretion assays, comparative genomics, and behavioral bioassays. Immunoreactive seductin was detected in eluates of egg cordons, indicating that seductin was secreted onto the cordon during egg laying. Aplysia brasiliana seductin was 94% identical to its A.californica homolog. In T-maze attraction assays, the combination of attractin and seductin was significantly attractive to potential mates, whereas either protein alone was not. Data from this and previous studies support the hypothesis that seductin is a water-borne protein pheromone that acts in concert with attractin, enticin, and temptin to attract Aplysia to form and maintain mating aggregations"
Keywords:		"Amino Acid Sequence Animals Aplysia Blotting, Western DNA Primers/chemistry DNA, Complementary/metabolism Electrophoresis, Polyacrylamide Gel Female Gene Library Glutathione Transferase/metabolism Glycoproteins/metabolism/*physiology Immunoblotting Insect;"
Notes:		"MedlineCummins, Scott F Nichols, Amy E Warso, Carrie J Nagle, Gregg T eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2005/01/18 Peptides. 2005 Mar; 26(3):351-9. doi: 10.1016/j.peptides.2004.10.024"