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Molecules

Title:		Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in Osmanthus fragrans
Author(s):		Yang XL; Li HY; Yue YZ; Ding WJ; Xu C; Shi TT; Chen GW; Wang LG;
Address:		"College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China. yangxl339@sina.com. College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China. tarashiyumi@163.com. College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China. yueyuanzheng@njfu.edu.cn. College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China. ruguoaiduideren@163.com. College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China. xc127@foxmail.com. College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China. shitingting313@163.com. College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China. 18694976599@163.com. College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China. wlg@njfu.com.cn. College of Forestry, Nanjing Forestry University, Nanjing 210037, China. wlg@njfu.com.cn"
Journal Title:		Molecules
Year:		2018
Volume:		20180702
Issue:		7
Page Number:		-
DOI:		10.3390/molecules23071604
ISSN/ISBN:		1420-3049 (Electronic) 1420-3049 (Linking)
Abstract:		"Osmanthus fragrans, or 'RiXiangGui', is an ornamental, woody, evergreen plant that is cultivated widely because it blooms recurrently and emits a strong fragrance. Recently, the germplasm resources, classification, and aroma compositions of O. fragrans have been investigated. However, the molecular mechanisms of the floral scent formation and regulation have remained largely unknown. To obtain a global perspective on the molecular mechanism of the aroma formation during blooming, nine RNA Sequencing (RNA-Seq) libraries were constructed from three flowering stages: The initial, full, and final flowering stage. In short, a total of 523,961,310 high-quality clean reads were assembled into 136,611unigenes, with an average sequence length of 792 bp. About 47.43% of the unigenes (64,795) could be annotated in the NCBI non-redundant protein database. A number of candidate genes were identified in the terpenoid metabolic pathways and 1327 transcription factors (TFs), which showed differential expression patterns among the floral scent formation stages, were also identified, especially OfMYB1, OfMYB6, OfWRKY1, and OfWRKY3, which could play critical roles in the floral scent formation. These results indicated that the floral scent formation of O. fragrans was a very complex process which involved a large number of TFs. This study provides reliable resources for further studies of the O.fragrans floral scent formation"
Keywords:		"Flowers/genetics Gene Expression Profiling/methods Gene Expression Regulation, Plant/genetics Genes, Plant/genetics Molecular Sequence Annotation/methods Oleaceae/*genetics Plant Proteins/genetics Sequence Analysis, RNA/methods Transcription Factors/genet;"
Notes:		"MedlineYang, Xiu-Lian Li, Hai-Yan Yue, Yuan-Zheng Ding, Wen-Jie Xu, Chen Shi, Ting-Ting Chen, Gong-Wei Wang, Liang-Gui eng Switzerland 2018/07/14 Molecules. 2018 Jul 2; 23(7):1604. doi: 10.3390/molecules23071604"