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BMC Biol

Title:		Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes
Author(s):		Xia YH; Ding BJ; Dong SL; Wang HL; Hofvander P; Lofstedt C;
Address:		"Department of Biology, Lund University, Solvegatan 37, SE-22362, Lund, Sweden. Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivagen 4, SE-41296, Gothenburg, Sweden. Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, CN-210095, China. Department of Plant Breeding, Swedish University of Agricultural Sciences, P.O. Box 101, SE-23053, Alnarp, Sweden. Department of Biology, Lund University, Solvegatan 37, SE-22362, Lund, Sweden. christer.lofstedt@biol.lu.se"
Journal Title:		BMC Biol
Year:		2022
Volume:		20220331
Issue:		1
Page Number:		80 -
DOI:		10.1186/s12915-022-01281-8
ISSN/ISBN:		1741-7007 (Electronic) 1741-7007 (Linking)
Abstract:		"BACKGROUND: Using genetically modified plants as natural dispensers of insect pheromones may eventually become part of a novel strategy for integrated pest management. RESULTS: In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem borer Chilo suppressalis (Walker) by heterologous expression in Saccharomyces cerevisiae and Nicotiana benthamiana, including two desaturase genes CsupYPAQ and CsupKPSE and a reductase gene CsupFAR2. Subsequently, we co-expressed CsupYPAQ and CsupFAR2 together with the previously characterized moth desaturase Atr?Os11 in N. benthamiana. This resulted in the production of (Z)-11-hexadecenol together with (Z)-11-hexadecenal, the major pheromone component of C. suppressalis. Both compounds were collected from the transformed N. benthamiana headspace volatiles using solid-phase microextraction. We finally added the expression of a yeast acetyltransferase gene ATF1 and could then confirm also (Z)-11-hexadecenyl acetate release from the plant. CONCLUSIONS: Our results pave the way for stable transformation of plants to be used as biological pheromone sources in different pest control strategies"
Keywords:		Animals *Moths/genetics Pheromones/metabolism *Sex Attractants Tobacco/genetics Acetyltransferase Alcohol oxidation Fatty acyl desaturases Fatty acyl reductase Functional characterization Heterologous expression systems Pheromone-releasing plants;
Notes:		"MedlineXia, Yi-Han Ding, Bao-Jian Dong, Shuang-Lin Wang, Hong-Lei Hofvander, Per Lofstedt, Christer eng Research Support, Non-U.S. Gov't England 2022/04/02 BMC Biol. 2022 Mar 31; 20(1):80. doi: 10.1186/s12915-022-01281-8"