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Acta Crystallogr D Biol Crystallogr

Title:		Structure of rat odorant-binding protein OBP1 at 1.6 A resolution
Author(s):		White SA; Briand L; Scott DJ; Borysik AJ;
Address:		"School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, England"
Journal Title:		Acta Crystallogr D Biol Crystallogr
Year:		2009
Volume:		20090418
Issue:		Pt 5
Page Number:		403 - 410
DOI:		10.1107/S090744490900420X
ISSN/ISBN:		1399-0047 (Electronic) 0907-4449 (Linking)
Abstract:		"The nasal mucosa is a specialist interfacial region sandwiched between the olfactory system and the gaseous chemical milieu. In mammals and insects, this region is rich in odorant-binding proteins that are thought to aid olfaction by assisting mass transfer of the many different organoleptic compounds that make up the olfactory landscape. However, in mammals at least, our grasp on the exact function of odorant-binding proteins is tentative and better insight into the role of these proteins is warranted, not least because of their apparent significance in the olfactory systems of insects. Here, the crystal structure of rat odorant-binding protein 1 is reported at 1.6 A resolution. This protein is one of the best-characterized mammalian odorant-binding proteins and only the third such protein structure to be solved at high resolution. The protein was crystallized in the holo form and contains an unidentifiable ligand that is probably an artefact from the Pichia pastoris expression system. Comparisons are made between this structure and a modelled OBP1 structure produced using the crystal structure of aphrodisin as a template. Comparisons are also made between OBP1 and the other two rat OBP subtypes, for which crystallographic data are unavailable. Interestingly, we also show that OBP1 is monomeric, which is in contrast to its previous assignment"
Keywords:		"Amino Acid Sequence Animals Binding Sites Crystallography, X-Ray DNA-Binding Proteins/chemistry Lipocalins/chemistry Models, Molecular Molecular Sequence Data Pheromones/chemistry Protein Conformation Proteins/chemistry Rats/*metabolism Receptors, Odorant;"
Notes:		"MedlineWhite, Scott A Briand, Loic Scott, David J Borysik, Antoni J eng BB/D522870/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Comparative Study 2009/04/25 Acta Crystallogr D Biol Crystallogr. 2009 May; 65(Pt 5):403-10. doi: 10.1107/S090744490900420X. Epub 2009 Apr 18"