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J Microbiol Methods


Title:Use of gas chromatography-mass spectrometry/solid phase microextraction for the identification of MVOCs from moldy building materials
Author(s):Wady L; Bunte A; Pehrson C; Larsson L;
Address:"Department of Medical Microbiology, Dermatology and Infection, University of Lund, Solvegatan 23, Sweden"
Journal Title:J Microbiol Methods
Year:2003
Volume:52
Issue:3
Page Number:325 - 332
DOI: 10.1016/s0167-7012(02)00190-2
ISSN/ISBN:0167-7012 (Print) 0167-7012 (Linking)
Abstract:"Gas chromatography-mass spectrometry/solid phase microextraction (GC-MS/SPME) was applied to identify microbial volatile organic compounds (MVOCs) in water-damaged, mold-infested building materials (gypsum board papers (n=2), mineral wool, and masonite) and in cultivated molds (Aspergillus penicillioides, Stachybotrys chartarum, and Chaetomium globosum). Three SPME fibers (65-microm PDMS-DVB, 75-microm Carboxen-PDMS, and 70-microm Carbowax-stableflex) designed for automated injection were used of which the latter showed best performance. A number of previously reported MVOCs were detected both in the building materials and the cultivated molds. In addition, methyl benzoate was identified both in the S. chartarum and A. penicillioides cultures and in the building materials. SPME combined with GC-MS may be a useful method for the determination of MVOCs emitted from mold-infested building materials"
Keywords:"Air Pollution, Indoor/*analysis Aspergillus/chemistry/growth & development/isolation & purification Benzoates/analysis Chaetomium/chemistry/growth & development/isolation & purification Fungi/*chemistry Gas Chromatography-Mass Spectrometry/*methods Hot Te;"
Notes:"MedlineWady, Loay Bunte, Annicka Pehrson, Christina Larsson, Lennart eng Evaluation Study Research Support, Non-U.S. Gov't Netherlands 2003/01/18 J Microbiol Methods. 2003 Mar; 52(3):325-32. doi: 10.1016/s0167-7012(02)00190-2"

 
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