| Title: | Root bacterial endophytes confer drought resistance and enhance expression and activity of a vacuolar H(+) -pumping pyrophosphatase in pepper plants |
| Author(s): | Vigani G; Rolli E; Marasco R; Dell'Orto M; Michoud G; Soussi A; Raddadi N; Borin S; Sorlini C; Zocchi G; Daffonchio D; |
| Address: | "Department of Life Sciences and Systems Biology, University of Turin, Plant Physiology Unit, 10135, Turin, Italy. Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, 20133, Milan, Italy. Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia. Department of Agricultural and Environmental Sciences, Production, Landscape, Agroenergy (DISAA), University of Milan, 20133, Milan, Italy. Department of Civil, Alma Mater Studiorum University of Bologna, Chemical, Environmental and Materials Engineering (DICAM), Bologna, Italy" |
| ISSN/ISBN: | 1462-2920 (Electronic) 1462-2912 (Linking) |
| Abstract: | "It has been previously shown that the transgenic overexpression of the plant root vacuolar proton pumps H(+) -ATPase (V-ATPase) and H(+) -PPase (V-PPase) confer tolerance to drought. Since plant-root endophytic bacteria can also promote drought tolerance, we hypothesize that such promotion can be associated to the enhancement of the host vacuolar proton pumps expression and activity. To test this hypothesis, we selected two endophytic bacteria endowed with an array of in vitro plant growth promoting traits. Their genome sequences confirmed the presence of traits previously shown to confer drought resistance to plants, such as the synthesis of nitric oxide and of organic volatile organic compounds. We used the two strains on pepper (Capsicuum annuum L.) because of its high sensitivity to drought. Under drought conditions, both strains stimulated a larger root system and enhanced the leaves' photosynthetic activity. By testing the expression and activity of the vacuolar proton pumps, H(+) -ATPase (V-ATPase) and H(+) -PPase (V-PPase), we found that bacterial colonization enhanced V-PPase only. We conclude that the enhanced expression and activity of V-PPase can be favoured by the colonization of drought-tolerance-inducing bacterial endophytes" |
| Notes: | "PublisherVigani, Gianpiero Rolli, Eleonora Marasco, Ramona Dell'Orto, Marta Michoud, Gregoire Soussi, Asma Raddadi, Noura Borin, Sara Sorlini, Claudia Zocchi, Graziano Daffonchio, Daniele eng RBIN047MBH/Italian MIUR FIRB/ 245746/European Community's Seventh Framework Programme/ King Abdullah University of Science and Technology/ Universita degli Studi di Milano/ DeFENS/ Regione Lombardia/ England 2018/05/23 Environ Microbiol. 2018 May 22. doi: 10.1111/1462-2920.14272" |
