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Insect Biochem Mol Biol

Title:		Pheromone biosynthetic pathways: PBAN-regulated rate-limiting steps and differential expression of desaturase genes in moth species
Author(s):		Tsfadia O; Azrielli A; Falach L; Zada A; Roelofs W; Rafaeli A;
Address:		"Institute for Technology and Storage of Agricultural Products, ARO, Volcani Center, Bet Dagan 50250, Israel"
Journal Title:		Insect Biochem Mol Biol
Year:		2008
Volume:		20080205
Issue:		5
Page Number:		552 - 567
DOI:		10.1016/j.ibmb.2008.01.005
ISSN/ISBN:		0965-1748 (Print) 0965-1748 (Linking)
Abstract:		"We combine the use of labeled precursors with enzyme inhibitors to decipher the biosynthetic pathway of pheromone biosynthesis and the rate-limiting step/s that are regulated by pheromone biosynthesis activating neuropeptide (PBAN). We demonstrate that Plodia interpunctella is able to utilize hexadecanoic acid, and to a lesser extent tetradecanoic acid, for the biosynthesis of the main pheromone component (Z,E)-9,12-tetradecadienyl acetate. This indicated that the main pathway involves a Delta11 desaturase, chain shortening, followed by a Delta12 desaturase, but that a functional Delta9 desaturase could also be utilized. Using reverse transcription-quantitative real-time polymerase chain reaction (RT-QPCR) we distinguish two out of nine possible desaturase gene transcripts in P. interpunctella that are expressed at the highest levels. The rate-limiting step for PBAN-stimulation was studied in two moth species so as to compare the biosynthesis of a diene (P. interpunctella) and a monoene (Helicoverpa armigera) main pheromone component. In both species, incorporation of label from the (13)C sodium acetate precursor was activated by PBAN whereas no stimulatory action was observed in the incorporation of the precursors: (13)C malonyl coenzyme A; hexadecanoic 16,16,16-(2)H(3) or tetradecanoic 14,14,14-(2)H(3) acids. The acetyl coenzyme A carboxylase (ACCase) inhibitor, Tralkoxydim, inhibited the PBAN-stimulation of incorporation of stable isotope whereas the fatty-acyl reductase inhibitor, Mevastatin, failed to influence the stimulatory action of PBAN. These results provide irrefutable support to the hypothesis that PBAN affects the production of malonyl coenzyme A from acetate by the action of ACCase in the pheromone glands of these moths"
Keywords:		"Animals Carbon Isotopes/metabolism DNA, Complementary Deuterium/metabolism Fatty Acid Desaturases/genetics/*metabolism Female Gene Expression Male Malonyl Coenzyme A/metabolism Moths/enzymology/genetics/*metabolism Myristic Acid/metabolism Neuropeptides/*;"
Notes:		"MedlineTsfadia, Oren Azrielli, Avi Falach, Lily Zada, Anat Roelofs, Wendell Rafaeli, Ada eng Research Support, U.S. Gov't, Non-P.H.S. England 2008/04/15 Insect Biochem Mol Biol. 2008 May; 38(5):552-67. doi: 10.1016/j.ibmb.2008.01.005. Epub 2008 Feb 5"