| Title: | "Site of pheromone biosynthesis and isolation of HMG-CoA reductase cDNA in the cotton boll weevil, Anthonomus grandis" |
| Author(s): | Taban AH; Fu J; Blake J; Awano A; Tittiger C; Blomquist GJ; |
| Address: | "Department of Biochemistry and Molecular Biology, University of Nevada, Reno, 89557, USA" |
| Journal Title: | Arch Insect Biochem Physiol |
| ISSN/ISBN: | 0739-4462 (Print) 0739-4462 (Linking) |
| Abstract: | "Isolated gut tissue from male cotton boll weevil, Anthonomus grandis (Coleoptera: Curculionidae), incorporated radiolabeled acetate into components that co-eluted with monoterpenoid pheromone components on HPLC. This demonstrates that pheromone components of male A. grandis are produced de novo and strongly suggests that pheromone biosynthesis occurs in gut tissue. A central enzyme in isoprenoid biosynthesis is 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-R), and a full-length HMG-R cDNA was isolated from A. grandis. The predicted translation product was 54 and 45% identical to HMG-R from Ips paraconfusus and Drosophila melanogaster, respectively. HMG-R gene expression gradually increased with age in male A. grandis, which correlates with pheromone production. However, topical application of JH III did not significantly increase HMG-R mRNA levels" |
| Keywords: | "Age Factors Amino Acid Sequence Animals Base Sequence Blotting, Northern Chromatography, High Pressure Liquid Cluster Analysis DNA Primers DNA, Complementary/genetics Digestive System/*metabolism *Gene Expression Hydroxymethylglutaryl CoA Reductases/*gene;" |
| Notes: | "MedlineTaban, A Huma Fu, Jessica Blake, Jacob Awano, Ami Tittiger, Claus Blomquist, Gary J eng Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2006/08/26 Arch Insect Biochem Physiol. 2006 Aug; 62(4):153-63. doi: 10.1002/arch.20125" |
