| Title: | Bio-sniffer (gas-phase biosensor) with secondary alcohol dehydrogenase (S-ADH) for determination of isopropanol in exhaled air as a potential volatile biomarker |
| Author(s): | Chien PJ; Suzuki T; Tsujii M; Ye M; Toma K; Arakawa T; Iwasaki Y; Mitsubayashi K; |
| Address: | "Graduate school of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-35 Yamate-Cho,Yamate-Cho, Suita-Shi, Osaka, 564-0836 Japan. Graduate school of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. Electronic address: m.bdi@tmd.ac.jp" |
| DOI: | 10.1016/j.bios.2016.12.050 |
| ISSN/ISBN: | 1873-4235 (Electronic) 0956-5663 (Linking) |
| Abstract: | "Exhaled breath analysis has attracted lots of researchers attention in the past decades due to its advantages such as its non-invasive property and the possibility of continuous monitoring. In addition, several volatile organic compounds in breath have been identified as biomarkers for some diseases. Particularly, studies have pointed out that concentration of isopropanol (IPA) in exhaled air might relate with certain illnesses such as liver disease, chronic obstructive pulmonary (COPD), and lung cancer. In this study, a highly sensitive and selective biochemical gas sensor (bio-sniffer) for the breath IPA concentration determination was constructed and optimized. This bio-sniffer measures the concentration of IPA according to the fluorescence intensity of oxidized nicotinamide adenine dinucleotide (NADH), which was produced by an enzymatic reaction of secondary alcohol dehydrogenase (S-ADH). The NADH detection system employed an UV-LED as the excitation light, and a highly sensitive photomultiplier tube (PMT) as a fluorescence intensity detector. A gas-sensing region was developed using an optical fiber probe equipped with a flow-cell and enzyme immobilized membrane, and connected to the NADH measurement system. The calibration range of the IPA bio-sniffer was confirmed from 1ppb to 9060ppb that was comparable to other IPA analysis methods. The results of the analysis of breath IPA concentration in healthy subjects using the bio-sniffer showed a mean concentration of 16.0ppb, which was similar to other studies. These results have demonstrated that this highly sensitive and selective bio-sniffer could be used to measure the IPA in exhaled air, and it is expected to apply for breath IPA research and investigation of biomarkers for clinical diagnosis" |
| Keywords: | "2-Propanol/*analysis/metabolism Adult Alcohol Oxidoreductases/*metabolism Biosensing Techniques/*instrumentation Breath Tests/*instrumentation Enzymes, Immobilized/*metabolism Equipment Design Female Humans Male Yeasts/*enzymology Young Adult Biosensor Ga;" |
| Notes: | "MedlineChien, Po-Jen Suzuki, Takuma Tsujii, Masato Ye, Ming Toma, Koji Arakawa, Takahiro Iwasaki, Yasuhiko Mitsubayashi, Kohji eng England 2017/01/04 Biosens Bioelectron. 2017 May 15; 91:341-346. doi: 10.1016/j.bios.2016.12.050. Epub 2016 Dec 22" |
