« Previous AbstractA role for unsaturated fatty acids in mitochondrial movement and inheritance    Next Abstract"A sensitive analytical method for quantifying petromyzonol sulfate in water as a potential tool for population monitoring of the southern pouched lamprey, Geotria australis, in New Zealand streams" »

J Chem Ecol

Title:		"A rapid, sensitive, and selective method for quantitation of lamprey migratory pheromones in river water"
Author(s):		Stewart M; Baker CF; Cooney T;
Address:		"National Institute of Water and Atmospheric Research, Hamilton, New Zealand. m.stewart@niwa.co.nz"
Journal Title:		J Chem Ecol
Year:		2011
Volume:		20111111
Issue:		11
Page Number:		1203 - 1207
DOI:		10.1007/s10886-011-0029-y
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Linking)
Abstract:		"The methodology of using fish pheromones, or chemical signatures, as a tool to monitor or manage species of fish is rapidly gaining popularity. Unequivocal detection and accurate quantitation of extremely low concentrations of these chemicals in natural waters is paramount to using this technique as a management tool. Various species of lamprey are known to produce a mixture of three important migratory pheromones; petromyzonol sulfate (PS), petromyzonamine disulfate (PADS), and petromyzosterol disulfate (PSDS), but presently there are no established robust methods for quantitation of all three pheromones. In this study, we report a new, highly sensitive and selective method for the rapid identification and quantitation of these pheromones in river water samples. The procedure is based on pre-concentration, followed by liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis. The method is fast, with unambiguous pheromone determination. Practical quantitation limits of 0.25 ng/l were achieved for PS and PADS and 2.5 ng/l for PSDS in river water, using a 200-fold pre-concentration, However, lower quantitation limits can be achieved with greater pre-concentration. The methodology can be modified easily to include other chemicals of interest. Furthermore, the pre-concentration step can be applied easily in the field, circumventing potential stability issues of these chemicals"
Keywords:		"*Animal Migration Animals Cholestanes/analysis/chemistry/metabolism Cholic Acids/analysis/chemistry/metabolism Chromatography, Liquid/*methods *Lampreys Pheromones/*analysis/chemistry/metabolism Pyrrolidinones/analysis/chemistry/metabolism Reproducibility;"
Notes:		"MedlineStewart, Michael Baker, Cindy F Cooney, Terry eng 2011/11/15 J Chem Ecol. 2011 Nov; 37(11):1203-7. doi: 10.1007/s10886-011-0029-y. Epub 2011 Nov 11"