| Title: | Quantification of Seven Terpenes in Human Serum by Headspace Solid-Phase Microextraction-Gas Chromatography-Tandem Mass Spectrometry |
| Author(s): | Silva LK; Espenship MF; Newman CA; Blount BC; De Jesus VR; |
| Address: | "Division of Laboratory Sciences, National Center for Environmental Health, U.S. Centers for Disease Control and Prevention (CDC), Atlanta, Georgia 30341, United States" |
| ISSN/ISBN: | 1520-5851 (Electronic) 0013-936X (Linking) |
| Abstract: | "Terpenes are a class of volatile organic hydrocarbons commonly produced by vegetation and released into the atmosphere. These compounds are responsible for the scents of pine forests, citrus fruits, and some flowers. Human terpene exposure can come from inhalation, diet, smoking, and more recently, using e-cigarettes. Terpenes are present in tobacco smoke and are used as flavor chemicals in e-liquids. The health effects of terpenes are not widely known, though several studies have suggested that they may prove useful in future medical applications. We have developed a novel, high-throughput method of quantifying seven terpenes (alpha-pinene, beta-pinene, beta-myrcene, 3-carene, limonene, beta-caryophyllene, and alpha-humulene) in human serum to aid human-exposure investigations. This method employs headspace sampling using solid-phase microextraction (SPME) coupled to gas chromatography-tandem mass spectrometry to detect and quantify five monoterpenes and two sesquiterpenes in the low parts-per-trillion to low parts-per-billion range. The intraday and interday variability (percent error) of the method are |
| Keywords: | *Electronic Nicotine Delivery Systems Gas Chromatography-Mass Spectrometry Humans *Solid Phase Microextraction Tandem Mass Spectrometry Terpenes/analysis; |
| Notes: | "MedlineSilva, Lalith K Espenship, Michael F Newman, Cody A Blount, Benjamin C De Jesus, Victor R eng 2020/09/16 Environ Sci Technol. 2020 Nov 3; 54(21):13861-13867. doi: 10.1021/acs.est.0c03269. Epub 2020 Oct 12" |
