| Title: | Volatomic pattern of breast cancer and cancer-free tissues as a powerful strategy to identify potential biomarkers |
| Author(s): | Silva C; Perestrelo R; Silva P; Capelinha F; Tomas H; Camara JS; |
| Address: | "CQM, Centro de Quimica da Madeira, Universidade da Madeira, Campus da Penteada, 9020-105 Funchal, Portugal. SESARAM, EPE. Hospital Dr. Nelio Mendonca, Servico de Anatomia Patologica, Avenida Luis de Camoes, n degrees 57-9004-514 Funchal, Portugal. jsc@staff.uma.pt. CQM, Centro de Quimica da Madeira, Universidade da Madeira, Campus da Penteada, 9020-105 Funchal, Portugal and Faculdade de Ciencias Exatas e da Engenharia, Universidade da Madeira, Campus da Penteada, 9020-105 Funchal, Portugal" |
| ISSN/ISBN: | 1364-5528 (Electronic) 0003-2654 (Linking) |
| Abstract: | "Breast cancer (BC), ranked as the fifth amongst all cancers, remains at the top of women's cancers worldwide followed by colorectal, lung, cervix, and stomach cancers. The main handicap of most of the screening/diagnostic methods is based on their low sensitivity and specificity and the invasive behavior of most sampling procedures. The aim of this study was to establish the volatomic pattern of BC and cancer-free (CF) tissues (n = 30) from the same patients, as a powerful tool to identify a set of volatile organic metabolite (VOM) potential BC biomarkers which might be used together or complement with the traditional BC diagnostics strategies, through the integration of chromatographic data, obtained by solid-phase microextraction followed by gas chromatography-mass spectrometry (SPME/GC-qMS), with chemometric tools. A total of four metabolites: limonene, decanoic acid, acetic acid and furfural presented the highest contribution towards discrimination of BC and CF tissues (VIP > 1, p < 0.05). The discrimination efficiency and accuracy of BC tissue metabolites was ascertained by ROC curve analysis that allowed the identification of some metabolites with high sensitivity and specificity. The results obtained with this approach suggest the possibility of identifying endogenous metabolites as a platform to find potential BC biomarkers and pave the way to investigate the related metabolomic pathways in order to improve BC diagnostic tools. Moreover, deeper investigations could unravel novel mechanistic insights into the disease pathophysiology" |
| Keywords: | "Acetic Acid/analysis Adult Aged Aged, 80 and over Biomarkers, Tumor/*analysis Breast Neoplasms/*diagnosis Decanoic Acids/analysis Female Furaldehyde/analysis Gas Chromatography-Mass Spectrometry Humans Limonene/analysis Metabolomics/methods Middle Aged Mu;" |
| Notes: | "MedlineSilva, Catarina Perestrelo, Rosa Silva, Pedro Capelinha, Filipa Tomas, Helena Camara, Jose S eng England 2019/05/31 Analyst. 2019 Jul 21; 144(14):4153-4161. doi: 10.1039/c9an00263d. Epub 2019 May 30" |
