| Title: | "Purification, cloning and characterisation of odorant- and pheromone-binding proteins from pig nasal epithelium" |
| Author(s): | Scaloni A; Paolini S; Brandazza A; Fantacci M; Bottiglieri C; Marchese S; Navarrini A; Fini C; Ferrara L; Pelosi P; |
| Address: | "I.A.B.B.A.M.-Centro Internazionale Servizi di Spettrometria di Massa National Research Council, Napoli, Italy" |
| ISSN/ISBN: | 1420-682X (Print) 1420-682X (Linking) |
| Abstract: | "Two distinct classes of lipocalin isoforms (OBP-IIs and OBP-IIIs) were purified and identified from porcine nasal mucosa of male and female individuals. Using primers designed on their N-terminal sequence, the complete primary structures of the mature polypeptides were determined. Mass spectrometry analysis confirmed the identity of the cDNA-derived sequences and provided information regarding their post-translational modifications. These species strongly resemble a lipocalin expressed by von Ebner's gland and salivary lipocalins carrying sex-specific pheromones secreted only by the boar's submaxillary glands. Both OBP-IIs and OBP-IIIs present two cysteines paired in a disulphide bond; the remaining residues occur in a reduced form. In addition, OBP-IIIs are heavily glycosylated and markedly different in their glycan moiety from the salivary lipocalins. A three-dimensional model is proposed based on protein species with known structure. Like salivary lipocalins, OBP-IIIs bind a number of odorant molecules, with highest affinity for the specific pheromone 5alpha-androst-16-en-3-one. The high similarity between OBPs from the nasal area and lipocalins from secretory glands suggests a common function in binding the same pheromonal ligands, the latter carrying chemical messages into the environment the former delivering them to specific receptors" |
| Keywords: | "Amino Acid Sequence Androstenes/metabolism Animals Carrier Proteins/*chemistry/genetics/*isolation & purification/metabolism Cloning, Molecular Epithelium/*chemistry Female Gas Chromatography-Mass Spectrometry Glycosylation *Insect Proteins Ligands Lipoca;" |
| Notes: | "MedlineScaloni, A Paolini, S Brandazza, A Fantacci, M Bottiglieri, C Marchese, S Navarrini, A Fini, C Ferrara, L Pelosi, P eng Research Support, Non-U.S. Gov't Switzerland 2001/07/05 Cell Mol Life Sci. 2001 May; 58(5-6):823-34. doi: 10.1007/pl00000903" |
