| Title: | Optimization of headspace solid phase microextraction for the analysis of microbial volatile organic compounds emitted by fungi: Application to historical objects |
| Author(s): | Sawoszczuk T; Sygula-Cholewinska J; del Hoyo-Melendez JM; |
| Address: | "Cracow University of Economics, Faculty of Commodity Science, Department of Microbiology, Rakowicka 27 St., 31-510 Cracow, Poland. Electronic address: tomasz.sawoszczuk@uek.krakow.pl. Cracow University of Economics, Faculty of Commodity Science, Department of Microbiology, Rakowicka 27 St., 31-510 Cracow, Poland. Electronic address: sygulaj@uek.krakow.pl. Laboratory of Analysis and Non-Destructive Investigation of Heritage Objects, National Museum in Krakow, Pilsudskiego 14 St., 31-106 Cracow, Poland. Electronic address: jdelhoyo@muzeum.krakow.pl" |
| DOI: | 10.1016/j.chroma.2015.07.059 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "The main goal of this work was to optimize the SPME sampling method for measuring microbial volatile organic compounds (MVOCs) emitted by active molds that may deteriorate historical objects. A series of artificially aged model materials that resemble those found in historical objects was prepared and evaluated after exposure to four different types of fungi. The investigated pairs consisted of: Alternaria alternata on silk, Aspergillus niger on parchment, Chaetomium globosum on paper and wool, and Cladosporium herbarum on paper. First of all, a selection of the most efficient SPME fibers was carried out as there are six different types of fibers commercially available. It was important to find a fiber that absorbs the biggest number and the highest amount of MVOCs. The results allowed establishing and selecting the DVB/CAR/PDMS fiber as the most effective SPME fiber for this kind of an analysis. Another task was to optimize the time of MVOCs extraction on the fiber. It was recognized that a time between 12 and 24h is adequate for absorbing a high enough amount of MVOCs. In the last step the temperature of MVOCs desorption in the GC injection port was optimized. It was found that desorption at a temperature of 250 degrees C allowed obtaining chromatograms with the highest abundances of compounds. To the best of our knowledge this work constitutes the first attempt of the SPME method optimization for sampling MVOCs emitted by molds growing on historical objects" |
| Keywords: | Alternaria/metabolism Animals Aspergillus/metabolism Chaetomium/metabolism Cladosporium/metabolism Dimethylpolysiloxanes Fungi/*metabolism Gas Chromatography-Mass Spectrometry Paper Polyvinyls Silk Solid Phase Microextraction/*methods Temperature Volatile; |
| Notes: | "MedlineSawoszczuk, Tomasz Sygula-Cholewinska, Justyna del Hoyo-Melendez, Julio M eng Research Support, Non-U.S. Gov't Netherlands 2015/07/26 J Chromatogr A. 2015 Aug 28; 1409:30-45. doi: 10.1016/j.chroma.2015.07.059. Epub 2015 Jul 17" |
