| Title: | Thermal desorption comprehensive two-dimensional gas chromatography coupled to time of flight mass spectrometry for vapour phase mainstream tobacco smoke analysis |
| Author(s): | Savareear B; Brokl M; Wright C; Focant JF; |
| Address: | "Centre for Analytical Research and Technologies (CART), University of Liege, Belgium. Group Research and Development, British American Tobacco, Southampton, UK. Centre for Analytical Research and Technologies (CART), University of Liege, Belgium. Electronic address: jf.focant@ulg.ac.be" |
| DOI: | 10.1016/j.chroma.2017.10.013 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "A thermal desorption comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (TD-GCxGC-TOFMS) method has been developed for the analysis of mainstream tobacco smoke (MTS) vapour phase (VP). The selection process of the sample introduction approach involved comparing the results obtained from three different approaches: a) use of gas sampling bag followed by SPME (Tedlar((R))-SPME), b) gas sampling bag followed by TD (Tedlar((R))-TD), and c) sampling directly on TD sorbents (Direct-TD). Six different SPME fibers and six different TD sorbent beds were evaluated for the extraction capacities in terms of total number of peaks and related intensities or peak areas. The best results were obtained for the Direct-TD approach using Tenax TA/Carbograph1TD/Carboxen1003 sorbent tubes. The optimisation of TD tube desorption parameters was carried out using a face-centered central composite experimental design and resulted in the use of the Tenax TA/Carbograph 1TD/Carboxen 1003 sorbent with a 7.5min desorption time, a 60mL/min tube desorption flow, and a 250 degrees C tube desorption temperature. The optimised method was applied to the separation of MTS-VP constituents, with 665 analytes detected. The method precision ranged from 1% to 15% for over 99% of identified peak areas and from 0% to 3% and 0% to 1% for both first ((1)t(R)) and second ((2)t(R)) dimension retention times, respectively. The method was applied to the analyses of two cigarette types differing in their filter construction. Principal component analysis (PCA) allowed a clear differentiation of the studied cigarette types (PC1 describing 94% of the explained variance). Supervised Fisher ratio analysis permitted the identification of compounds responsible for the chemical differences between the two sample types. A set of 91 most relevant compounds was selected by applying a Fisher ratio cut-off approach and most of them were selectively removed by one of the cigarette filter types" |
| Keywords: | "Chemistry Techniques, Analytical/*methods *Gas Chromatography-Mass Spectrometry Gases/analysis Polymers/chemistry Principal Component Analysis Smoke/*analysis Tobacco/*chemistry Volatile Organic Compounds/analysis Comprehensive two-dimensional gas chromat;" |
| Notes: | "MedlineSavareear, Benjamin Brokl, Michal Wright, Chris Focant, Jean-Francois eng Netherlands 2017/10/17 J Chromatogr A. 2017 Nov 24; 1525:126-137. doi: 10.1016/j.chroma.2017.10.013. Epub 2017 Oct 6" |
