Title: | Selection and application of antifungal VOCs-producing yeasts as biocontrol agents of grey mould in fruits |
Author(s): | Ruiz-Moyano S; Hernandez A; Galvan AI; Cordoba MG; Casquete R; Serradilla MJ; Martin A; |
Address: | "Nutricion y Bromatologia, Escuela de Ingenierias Agrarias, Instituto Universitario de Recursos Agrarios (INURA), Universidad de Extremadura, Ctra. de Caceres s/n, 06007, Badajoz, Spain. Nutricion y Bromatologia, Escuela de Ingenierias Agrarias, Instituto Universitario de Recursos Agrarios (INURA), Universidad de Extremadura, Ctra. de Caceres s/n, 06007, Badajoz, Spain. Electronic address: ahernandez@unex.es. Area de Hortofruticultura, Centro de Investigaciones Finca La Orden-Valdesequera (CICYTEX), Autovia Madrid-Lisboa, s/n, 060187, Guadajira, Spain. Area de Vegetales, Instituto Tecnologico Agroalimentario de Extremadura (INTAEX), Centro de Investigaciones Cientificas y Tecnologicas de Extremadura (CICYTEX), Avenida Adolfo Suarez s/n, 06007, Badajoz, Spain" |
ISSN/ISBN: | 1095-9998 (Electronic) 0740-0020 (Linking) |
Abstract: | "Rotting caused by grey mould (Botrytis cinerea) is a concerning disease for numerous crops both pre- and postharvest stages. Application of antagonistic yeasts is a promising strategy for controlling grey mould incidence which could mitigate undesirable consequences of using synthetic fungicides. In this work, a screening for detection of yeasts isolated from figs producers of antifungal volatile organic compounds (VOCs) were performed by confrontation in double dishes systems. Eleven out of 34 yeasts confronted reduced B. cinerea growth parameter in vitro. This reduction was correlated (p = 0.050) with the production of 10 volatile compounds: two acids (acetic acid and octanoic acid), 7 esters (Ethyl propionate, n-Propyl acetate, Isobutyl acetate, 2-methylbutyl acetate, furfuryl acetate, phenylmethyl acetate, 2-phenylethyl acetate) and one ketone (Heptan-2-one). In bases on in vitro assay, Hanseniaspora uvarum 793 was applied to in vivo assays with strawberries and cherries. The reduction of incidence of B. cinerea in strawberries at 7 degrees C and 25 degrees C was 54.9 and 72.1% after 6 and 3 days, respectively. The reduction of incidence of B. cinerea in cherries at 7 degrees C and 25 degrees C was 48.9 and 45.6% after 5 and 4 days, respectively. These results showed that VOCs produced by Hanseniaspora uvarum 793 are effective in the control of incidence of Botrytis cinerea in fruits, being a potential alternative to chemical fungicide" |
Keywords: | "Botrytis/*drug effects/growth & development Ficus/microbiology Fragaria/microbiology Fruit/*microbiology Fungicides, Industrial/chemistry/metabolism/*pharmacology Hanseniaspora/drug effects/growth & development Plant Diseases/*microbiology/prevention & co;" |
Notes: | "MedlineRuiz-Moyano, Santiago Hernandez, Alejandro Galvan, Ana I Cordoba, Maria G Casquete, Rocio Serradilla, Manuel J Martin, Alberto eng England 2020/09/21 Food Microbiol. 2020 Dec; 92:103556. doi: 10.1016/j.fm.2020.103556. Epub 2020 Jun 6" |