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« Previous AbstractSpatial and temporal distribution of pheromone biosynthesis-activating neuropeptide in Helicoverpa (Heliothis) armigera using RIA and in vitro bioassay    Next AbstractSpatial distribution and differential expression of the PBAN receptor in tissues of adult Helicoverpa spp. (Lepidoptera: Noctuidae) »

Peptides


Title:Synthesis and biological activity of a photoaffinity-biotinylated pheromone-biosynthesis activating neuropeptide (PBAN) analog
Author(s):Rafaeli A; Gileadi C;
Address:"Department of Stored Products, Volcani Center, Bet Dagan, Israel. vtada@volcani.agri.gov.il"
Journal Title:Peptides
Year:1999
Volume:20
Issue:7
Page Number:787 - 794
DOI: 10.1016/s0196-9781(99)00063-7
ISSN/ISBN:0196-9781 (Print) 0196-9781 (Linking)
Abstract:"To study the mode of action of pheromone-biosynthesis activating neuropeptide (PBAN) at the receptor level and for receptor purification, we synthesized and tested the biologic properties of a photoaffinity biotinylated PBAN analog N-[N-(4-azido-tetrafluorobenzoyl)-biocytinyloxyl-succinimide (Atf-Bct-NHS-PBAN). The Atf-Bct-NHS-PBAN was separated from unreacted reagent and synthetic Hez-PBAN by high-performance liquid chromatography. Conjugated biotin was detected by using enzyme-linked assay as well as tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis. The biologic activity of purified Atf-Bct-NHS-PBAN was confirmed using both in vivo and in vitro pheromonotropic bioassays. These observations indicate that Atf-Bct-NHS-PBAN is a full agonist of PBAN action in pheromone glands and may be used to study PBAN receptors by employing avidin coupled to various reporter groups"
Keywords:"Affinity Labels/*chemical synthesis Animals Avidin Biological Assay Biotin Female In Vitro Techniques Male Moths Neuropeptides/*chemical synthesis/*pharmacology Pheromones/*biosynthesis Receptors, Neuropeptide/drug effects;"
Notes:"MedlineRafaeli, A Gileadi, C eng Research Support, Non-U.S. Gov't 1999/09/07 Peptides. 1999; 20(7):787-94. doi: 10.1016/s0196-9781(99)00063-7"

 
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