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Can J Microbiol


Title:Purification of sex pheromones specific for pMB1 and pMB2 plasmids of Enterococcus faecalis S-48
Author(s):Quirantes R; Galvez A; Valdivia E; Martin I; Martinez-Bueno M; Mendez E; Maqueda M;
Address:"Department of Microbiology, Faculty of Sciences, University of Granada, Spain"
Journal Title:Can J Microbiol
Year:1995
Volume:41
Issue:7
Page Number:629 - 632
DOI: 10.1139/m95-084
ISSN/ISBN:0008-4166 (Print) 0008-4166 (Linking)
Abstract:"The strain Enterococcus faecalis S-48 carries two large conjugative plasmids (pMB1 and pMB2) encoding for antagonistic substances. The pheromone response of these two plasmids was established by purifying the corresponding sex pheromones, using conventional reversed-phase columns. Plasmid pMB1 responds to pheromone cCF10. Plasmid pMB2 responds to a pheromone with an amino acid sequence identical to that of cPD1 (Phe-Leu-Val-Met-Phe-Leu-Ser-Gly). The two pheromone-responding plasmids coexist in a stable fashion in the wild-type strain E. faecalis S-48"
Keywords:"Amino Acid Sequence Bacterial Adhesion/drug effects Conjugation, Genetic/*drug effects/genetics Enterococcus faecalis/chemistry/*genetics Molecular Sequence Data Plasmids/*physiology Sequence Analysis Sex Attractants/*isolation & purification/pharmacology;"
Notes:"MedlineQuirantes, R Galvez, A Valdivia, E Martin, I Martinez-Bueno, M Mendez, E Maqueda, M eng Research Support, Non-U.S. Gov't Canada 1995/07/01 Can J Microbiol. 1995 Jul; 41(7):629-32. doi: 10.1139/m95-084"

 
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