| Title: | "Endophytic Trichoderma gamsii YIM PH30019: a promising biocontrol agent with hyperosmolar, mycoparasitism, and antagonistic activities of induced volatile organic compounds on root-rot pathogenic fungi of Panax notoginseng" |
| Author(s): | Chen JL; Sun SZ; Miao CP; Wu K; Chen YW; Xu LH; Guan HL; Zhao LX; |
| Address: | "School of Energy and Environment Science, Yunnan Normal University, Kunming, China; Key Laboratory of Microbial Diversity in South West China of Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, China. School of Energy and Environment Science, Yunnan Normal University, Kunming, China. Key Laboratory of Microbial Diversity in South West China of Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, China" |
| DOI: | 10.1016/j.jgr.2015.09.006 |
| ISSN/ISBN: | 1226-8453 (Print) 2093-4947 (Electronic) 1226-8453 (Linking) |
| Abstract: | "BACKGROUND: Biocontrol agents are regarded as promising and environmental friendly approaches as agrochemicals for phytodiseases that cause serious environmental and health problems. Trichoderma species have been widely used in suppression of soil-borne pathogens. In this study, an endophytic fungus, Trichoderma gamsii YIM PH30019, from healthy Panax notoginseng root was investigated for its biocontrol potential. METHODS: In vitro detached healthy roots, and pot and field experiments were used to investigate the pathogenicity and biocontrol efficacy of T. gamsii YIM PH30019 to the host plant. The antagonistic mechanisms against test phytopathogens were analyzed using dual culture, scanning electron microscopy, and volatile organic compounds (VOCs). Tolerance to chemical fertilizers was also tested in a series of concentrations. RESULTS: The results indicated that T. gamsii YIM PH30019 was nonpathogenic to the host, presented appreciable biocontrol efficacy, and could tolerate chemical fertilizer concentrations of up to 20%. T. gamsii YIM PH30019 displayed antagonistic activities against the pathogenic fungi of P. notoginseng via production of VOCs. On the basis of gas chromatography-mass spectrometry, VOCs were identified as dimethyl disulfide, dibenzofuran, methanethiol, ketones, etc., which are effective ingredients for antagonistic activity. T. gamsii YIM PH30019 was able to improve the seedlings' emergence and protect P. notoginseng plants from soil-borne disease in the continuous cropping field tests. CONCLUSION: The results suggest that the endophytic fungus T. gamsii YIM PH30019 may have a good potential as a biological control agent against notoginseng phytodiseases and can provide a clue to further illuminate the interactions between Trichoderma and phytopathogens" |
| Keywords: | Panax notoginseng Trichoderma gamsii antagonistic activity biocontrol agents mycoparasitism; |
| Notes: | "PubMed-not-MEDLINEChen, Jin-Lian Sun, Shi-Zhong Miao, Cui-Ping Wu, Kai Chen, You-Wei Xu, Li-Hua Guan, Hui-Lin Zhao, Li-Xing eng Korea (South) 2016/10/18 J Ginseng Res. 2016 Oct; 40(4):315-324. doi: 10.1016/j.jgr.2015.09.006. Epub 2015 Oct 22" |
