| Title: | Speciation of arsenic metabolites in the urine of occupational workers and experimental rats using an optimised hydride cold-trapping method |
| Author(s): | Ng JC; Johnson D; Imray P; Chiswell B; Moore MR; |
| Address: | "National Research Centre for Environmental Toxicology, Brisbane, Austalia. j.ng@mailbox.ug.edu.au" |
| ISSN/ISBN: | 0003-2654 (Print) 0003-2654 (Linking) |
| Abstract: | "A hydride cold-trapping technique was developed and optimised for the measurement of urinary arsenic metabolites. The analytical precision of the method was found to be 6.1, 4.0 and 4.8% (n = 5) for inorganic arsenic (ASi), monomethylarsonate (MMA) and dimethylarsinate (DMA), respectively, with recoveries close to 100%. The detection limits were 1.0, 1.3 and 3 ng for ASi, MMA and DMA, respectively. The method was then used to analyse urine samples obtained from three groups of workers for occupational exposure in three companies where copper chrome arsenate was used for timber treatment. The results were compared with those for a normal control group of laboratory workers. Arsenic and its metabolites were also measured in experimental rats given 5 mg As kg-1 body mass by oral gavage in the form of sodium arsenite, calcium arsenite or sodium arsenate. Occupational workers showed a significantly higher excretion of ASi. Up to two fold increases of urinary ASi excretion in rats compared with control rats were also observed in animals dosed with various forms of arsenicals. The method is suitable for the measurement of arsenic metabolites in urine of both humans and experimental animals" |
| Keywords: | "Animals;Animals Arsenic/toxicity/*urine Arsenicals/urine *Forestry Humans *Occupational Exposure Rats Risk Assessment Spectrophotometry, Atomic;" |
| Notes: | "MedlineNg, J C Johnson, D Imray, P Chiswell, B Moore, M R eng Research Support, Non-U.S. Gov't England 1998/08/26 Analyst. 1998 May; 123(5):929-33. doi: 10.1039/a707726b" |
