Title: | Revised model for Enterococcus faecalis fsr quorum-sensing system: the small open reading frame fsrD encodes the gelatinase biosynthesis-activating pheromone propeptide corresponding to staphylococcal agrd |
Author(s): | Nakayama J; Chen S; Oyama N; Nishiguchi K; Azab EA; Tanaka E; Kariyama R; Sonomoto K; |
Address: | "Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. nakayama@agr.kyushu-u.ac.jp" |
ISSN/ISBN: | 0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking) |
Abstract: | "Gelatinase biosynthesis-activating pheromone (GBAP) is an autoinducing peptide involved in Enterococcus faecalis fsr quorum sensing, and its 11-amino-acid sequence has been identified in the C-terminal region of the 242-residue deduced fsrB product (J. Nakayama et al., Mol. Microbiol. 41:145-154, 2001). In this study, however, we demonstrated the existence of fsrD, encoding the GBAP propeptide, which is in frame with fsrB but is translated independently of fsrB. It was also demonstrated that FsrB', an FsrD segment-truncated FsrB, functions as a cysteine protease-like processing enzyme to generate GBAP from FsrD. This revised model is consistent with the staphylococcal agr system" |
Keywords: | "Amino Acid Sequence Bacterial Proteins/genetics/metabolism/physiology Cysteine Endopeptidases/metabolism Enterococcus faecalis/*physiology Lactones/metabolism Molecular Sequence Data Mutagenesis, Site-Directed Open Reading Frames/*genetics Peptides, Cycli;" |
Notes: | "MedlineNakayama, Jiro Chen, Shengmin Oyama, Nozomi Nishiguchi, Kenzo Azab, Essam A Tanaka, Emi Kariyama, Reiko Sonomoto, Kenji eng Research Support, Non-U.S. Gov't 2006/09/19 J Bacteriol. 2006 Dec; 188(23):8321-6. doi: 10.1128/JB.00865-06. Epub 2006 Sep 15" |