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J Bacteriol

Title:		Role of metabolism of the mating pheromone in sexual differentiation of the heterobasidiomycete Rhodosporidium toruloides
Author(s):		Miyakawa T; Nishihara M; Tsuchiya E; Fukui S;
Address:
Journal Title:		J Bacteriol
Year:		1982
Volume:		151
Issue:		3
Page Number:		1184 - 1194
DOI:		10.1128/jb.151.3.1184-1194.1982
ISSN/ISBN:		0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking)
Abstract:		"A trypsin-type endopeptidase (Kamiya et al., Biochem. Biophys. Res. Commun. 94:855-860, 1980) responsible for the metabolism of rhodotorucine A, the farnesyl undecapeptide mating pheromone secreted by mating type A cells of Rhodosporidium toruloides, was biologically characterized. Metabolic activity was found to be present exclusively on the cell surface of the pheromone target cell. The activity was highly specific to the pheromone, and a biologically inactive analog which has the complete amino acid sequence of rhodotorucine A but lacks the farnesyl residue was not metabolized by intact cells. Pheromone metabolism was inhibited by trypsin substrates such as tosyl-L-arginine methyl ester. The presence of tosyl-L-arginine methyl ester strongly inhibited the sexual differentiation induced by the pheromone at a concentration which did not affect the vegetative growth of R. toruloides. Pheromone-induced sexual differentiation was also strongly inhibited by a metabolizable analog, rhodotorucine A S-oxide, but not by a non-metabolizable one. In mutants defective in early processes of mating, the decrease in the pheromone metabolic activity correlated well with the extent of loss of sensitivity to the pheromone. Both the pheromone metabolism and the capacity for sexual differentiation of a sterile mutant were restored concomitantly with reversion from the sterile to the fertile phenotype. These results suggested that metabolism of the mating pheromone plays an essential role in the process of sexual differentiation in R. toruloides"
Keywords:		Basidiomycota/*metabolism Benzoylarginine-2-Naphthylamide/pharmacology Cell Membrane/enzymology Endopeptidases/*metabolism Farnesol/*analogs & derivatives/metabolism Kinetics Mutation Oligopeptides/*metabolism Tosylarginine Methyl Ester/pharmacology Ustil;
Notes:		"MedlineMiyakawa, T Nishihara, M Tsuchiya, E Fukui, S eng Research Support, Non-U.S. Gov't 1982/09/01 J Bacteriol. 1982 Sep; 151(3):1184-94. doi: 10.1128/jb.151.3.1184-1194.1982"