| Title: | Role of ABC Proteins in Secondary Metabolism and Immune (=Defensive) Response in Seaweeds |
| Author(s): | Salgado LT; Oliveira LS; Echevarria-Lima J; Reis VM; Sudatti DB; Thompson FL; Pereira RC; |
| Address: | "Diretoria de Pesquisas, Instituto de Pesquisas Jardim Botanico do Rio de Janeiro, Rio de Janeiro 22460-030, Brazil. Departamento de Biotecnologia Marinha, Instituto de Estudos do Mar Almirante Paulo Moreira-IEAPM, Arraial do Cabo 28930-000, RJ, Brazil. Laboratorio de Imunologia Celular e Aplicada, Departamento de Imunologia, Instituto de Microbiologia Paulo Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil. Departamento de Biologia Marinha, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil. Departamento de Biologia Marinha, Instituto de Biologia, SAGE-COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil" |
| ISSN/ISBN: | 2073-4409 (Electronic) 2073-4409 (Linking) |
| Abstract: | "Laurencia seaweed species synthesize a broad range of secondary metabolites, mainly terpenes (e.g., elatol), exhibiting diverse ecological roles, such as defense against fouling and herbivores. Recently, an intricate cellular machinery was described concerning terpenes biosynthetic pathways, storage inside corps en cerise (CC), and regulated exocytosis in these species. But for seaweeds in general, the proteins involved in transmembrane transport of secondary metabolites remain unknown. Assays with Rhodamine-123 and cyclosporine A (CSA) revealed the presence of ABC transporters in CC membrane of Laurencia dendroidea. In vivo incubation assays with CSA resulted in CC morphological changes, reduced intracellular elatol concentrations, and increased biofouling cover on the seaweed surface. Cultivation assays in the presence of a marine pathogenic bacteria induced the expression of ABC proteins belonging to the subfamilies ABCB, ABCD, ABCF, and ABCG. The latter subfamily is known to be associated with the transport of plant terpenes. Our results shed new light on the role of ABC proteins in key mechanisms of the defensive system in seaweeds against fouling and herbivory" |
| Keywords: | Secondary Metabolism *ATP-Binding Cassette Transporters Cyclosporine *Seaweed Terpenes chemical defense fouling herbivory; |
| Notes: | "MedlineSalgado, Leonardo T Oliveira, Louisi S Echevarria-Lima, Juliana Reis, Vanessa M Sudatti, Daniela B Thompson, Fabiano L Pereira, Renato C eng Research Support, Non-U.S. Gov't Switzerland 2023/09/28 Cells. 2023 Sep 12; 12(18):2259. doi: 10.3390/cells12182259" |
