| Title: | The transcriptome of cis-jasmone-induced resistance in Arabidopsis thaliana and its role in indirect defence |
| Author(s): | Matthes MC; Bruce TJ; Ton J; Verrier PJ; Pickett JA; Napier JA; |
| Address: | "Biological Chemistry Department, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK" |
| DOI: | 10.1007/s00425-010-1244-4 |
| ISSN/ISBN: | 1432-2048 (Electronic) 0032-0935 (Linking) |
| Abstract: | "cis-jasmone (CJ) is a plant-derived chemical that enhances direct and indirect plant defence against herbivorous insects. To study the signalling pathway behind this defence response, we performed microarray-based transcriptome analysis of CJ-treated Arabidopsis plants. CJ influenced a different set of genes from the structurally related oxylipin methyl jasmonate (MeJA), suggesting that CJ triggers a distinct signalling pathway. CJ is postulated to be biosynthetically derived from jasmonic acid, which can boost its own production through transcriptional up-regulation of the octadecanoid biosynthesis genes LOX2, AOS and OPR3. However, no effect on these genes was detected by treatment with CJ. Furthermore, CJ-responsive genes were not affected by mutations in COI1 or JAR1, which are critical signalling components in MeJA response pathway. Conversely, a significant proportion of CJ-inducible genes required the three transcription factors TGA2, TGA5 and TGA6, as well as the GRAS regulatory protein SCARECROW-like 14 (SCL14), indicating regulation by a different pathway from the classical MeJA response. Moreover, the biological importance was demonstrated in that mutations in TGA2, 5, 6, SCL14 and the CJ-inducible gene CYP81D11 blocked CJ-induced attraction of the aphid parasitoid Aphidius ervi, demonstrating that these components play a key role in CJ-induced indirect defence. Collectively, our results identify CJ as a member of the jasmonates that controls indirect plant defence through a distinct signalling pathway" |
| Keywords: | "Arabidopsis/*drug effects/*genetics Arabidopsis Proteins/*genetics Basic-Leucine Zipper Transcription Factors/genetics Blotting, Northern Cyclopentanes/*pharmacology *Gene Expression Profiling Gene Expression Regulation, Plant/drug effects/genetics Nuclea;" |
| Notes: | "MedlineMatthes, Michaela C Bruce, Toby J A Ton, Jurriaan Verrier, Paul J Pickett, John A Napier, Johnathan A eng BB/E023959/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom BBS/E/C/00004949/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom (BB/E023959/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't Germany 2010/08/17 Planta. 2010 Oct; 232(5):1163-80. doi: 10.1007/s00425-010-1244-4. Epub 2010 Aug 14" |
