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Plant Mol Biol

Title:		The role of H(2)S in low temperature-induced cucurbitacin C increases in cucumber
Author(s):		Liu Z; Li Y; Cao C; Liang S; Ma Y; Liu X; Pei Y;
Address:		"School of Life Science, Shanxi University, Taiyuan, 030006, China. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing, 100000, China. Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing, 100000, China. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518124, China. School of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China. School of Life Science, Shanxi University, Taiyuan, 030006, China. peiyanxi@sxu.edu.cn"
Journal Title:		Plant Mol Biol
Year:		2019
Volume:		20190201
Issue:		6
Page Number:		535 - 544
DOI:		10.1007/s11103-019-00834-w
ISSN/ISBN:		1573-5028 (Electronic) 0167-4412 (Linking)
Abstract:		"In this study, we first linked the signal molecule H(2)S with cucurbitacin C, which can cause the bitter taste of cucumber leaves and fruit, and specifically discuss its molecular mechanism. Cucurbitacin C (CuC), a triterpenoid secondary metabolite, enhances the resistance of cucumber plants to pathogenic bacteria and insect herbivores, but results in bitter-tasting fruits. CuC can be induced in some varieties of cucumber on exposure to plant stressors. The gasotransmitter hydrogen sulfide (H(2)S) participates in multiple physiological processes relating to plant stress resistance. This study focused on the effect of H(2)S on low temperature-induced CuC synthesis in cucumber. The results showed that treatment of cucumber leaves at 4 degrees C for 12 h enhanced the content and production rate of H(2)S and increased the expression of genes encoding enzymes involved in H(2)S generation, Csa2G034800.1 (CsaLCD), Csa1G574800.1 (CsaDES1), and Csa1G574810.1 (CsaDES2). In addition, treatment at 4 degrees C or with exogenous H(2)S upregulated the expression of CuC synthetase-encoding genes and the resulting CuC content in cucumber leaves, whereas pretreatment with hypotaurine (HT, a H(2)S scavenger) before treatment at 4 degrees C offset these effects. In vitro, H(2)S could increase the S-sulfhydration level of His-Csa5G156220 and His-Csa5G157230 (both bHLH transcription factors), as well as their binding activity to the promoter of Csa6G088690, which encodes the key synthetase for CuC generation. H(2)S pretreatment enhanced the cucumber leaves resistance to the Phytophthora melonis. Together, these results demonstrated that H(2)S acts as a positive regulator of CuC synthesis as a result of the modification of proteins by S-sulfhydration, also providing indirect evidence for the role of H(2)S in improving the resistance of plants to abiotic stresses and biotic stresses by regulating the synthesis of secondary metabolites"
Keywords:		"*Cold Temperature Cucumis sativus/genetics/*metabolism/parasitology Disease Resistance/genetics Gene Expression Regulation, Plant/drug effects Genes, Plant/genetics Hydrogen Sulfide/*metabolism Phytophthora/pathogenicity Plant Leaves/metabolism Secondary;"
Notes:		"MedlineLiu, Zhiqiang Li, Yawen Cao, Chunyu Liang, Shan Ma, Yongshuo Liu, Xin Pei, Yanxi eng 31671605/National Natural Science Foundation/ QNJJ2017-06/Beijing Technology and Business University Youth Fund/ Netherlands 2019/02/02 Plant Mol Biol. 2019 Apr; 99(6):535-544. doi: 10.1007/s11103-019-00834-w. Epub 2019 Feb 1"