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J Chem Ecol

Title:		Pheromone binding to general odorant-binding proteins from the navel orangeworm
Author(s):		Liu Z; Vidal DM; Syed Z; Ishida Y; Leal WS;
Address:		"Department of Entomology, University of California-Davis, 1 Shields Ave, Davis, CA 95616, USA"
Journal Title:		J Chem Ecol
Year:		2010
Volume:		20100610
Issue:		7
Page Number:		787 - 794
DOI:		10.1007/s10886-010-9811-5
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Print) 0098-0331 (Linking)
Abstract:		"General odorant-binding proteins (GOBPs) of moths are postulated to be involved in the reception of semiochemicals other than sex pheromones, the so-called 'general odorants.' We have expressed two GOBPs, AtraGOBP1 and AtraGOBP2, which were previously isolated from the antennae of the navel orangeworm, Amyelois transitella. Surprisingly, these two proteins did not bind compounds that are known to attract adult moths, particularly females. The proper folding and functionality of the recombinant proteins was inferred from circular dichroism analysis and demonstration that both GOBPs bound nonanal in a pH-dependent manner. EAG experiments demonstrated that female attractants (1-phenylethanol, propionic acid phenyl ester, and isobutyric acid phenyl ester) are detected with high sensitivity by the antennae of day-0 to day-4 adult females, with response declining in older moths. The same age-dependence was shown for male antennae responding to constituents of the sex pheromone. Interestingly, AtraGOBP2 bound the major constituent of the sex pheromone, Z11Z13-16Ald, with affinity comparable to that shown by a pheromone-binding protein, AtraPBP1. The related alcohol bound to AtraPBP1 with higher affinity than to AtraGOBP2. AtraGOBP1 bound both ligands with low but nearly the same affinity"
Keywords:		"Animals Circular Dichroism Female Hydrogen-Ion Concentration Male Moths/*physiology Protein Binding Protein Folding Receptors, Odorant/*chemistry/genetics/metabolism Recombinant Proteins/chemistry/genetics/metabolism Sex Attractants/chemistry/*physiology;"
Notes:		"MedlineLiu, Zhao Vidal, Diogo M Syed, Zainulabeuddin Ishida, Yuko Leal, Walter S eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2010/06/11 J Chem Ecol. 2010 Jul; 36(7):787-94. doi: 10.1007/s10886-010-9811-5. Epub 2010 Jun 10"