| Title: | Standardization study of expiratory conditions for on-line breath testing by proton transfer reaction mass spectrometry |
| Author(s): | Li B; Zou X; Wang H; Lu Y; Shen C; Chu Y; |
| Address: | "Department of Thoracic Surgery, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China. Anhui Province Key Laboratory of Medical Physics and Technology, Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, 230031, China. Electronic address: xzou@cmpt.ac.cn. Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui, 230031, China. Anhui Province Key Laboratory of Medical Physics and Technology, Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, 230031, China. Anhui Province Key Laboratory of Medical Physics and Technology, Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, 230031, China. Electronic address: chyshen@aiofm.ac.cn" |
| ISSN/ISBN: | 1096-0309 (Electronic) 0003-2697 (Linking) |
| Abstract: | "Breath testing is a noninvasive method with potential for diagnosing cancers and has been regarded as one of the research hotspots in metabolomics. In the conventional breath sampling process, however, degradation of exhaled metabolites and introduction of impurities from sampling bags or tubes limit the development of breath research. To solve this problem, we previously developed an on-line breath sampling system, which can directly deliver exhaled gases from the mouth to the proton transfer reaction mass spectrometry (PTR-MS) breath analysis instrument. To establish a standard expiratory method for this system, four parameters that may affect the concentrations of exhaled volatile organic compounds (VOCs) were studied. We found inhaled gas volume, breath holding time, mouth rinsing, and ambient air all affected the exhaled VOCs. In particular, the breath holding time and mouth rinsing significantly affected the VOCs which originate from the oral cavity. Therefore, these four parameters should be taken into account in future on-line breath testing" |
| Keywords: | Breath Tests Female Humans Male *Mass Spectrometry *Protons Volatile Organic Compounds/*analysis Breath testing On-line Ptr-ms Standardization; |
| Notes: | "MedlineLi, Baozhong Zou, Xue Wang, Hongmei Lu, Yan Shen, Chengyin Chu, Yannan eng Clinical Trial Research Support, Non-U.S. Gov't 2019/06/25 Anal Biochem. 2019 Sep 15; 581:113344. doi: 10.1016/j.ab.2019.113344. Epub 2019 Jun 21" |
