| Title: | Production system influences volatile biomarkers in tomato |
| Author(s): | Lee JHJ; Jayaprakasha GK; Rush CM; Crosby KM; Patil BS; |
| Address: | "Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, 1500 Research Parkway, A120, College Station, TX, 77845-2119, USA. Plant Pathology, Texas A&M AgriLife Research, and Extension, Amarillo Research & Extension Center, 6500 Amarillo Boulevard West, Amarillo, TX, 79106, USA. Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, 1500 Research Parkway, A120, College Station, TX, 77845-2119, USA. k-crosby@tamu.edu. Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, 1500 Research Parkway, A120, College Station, TX, 77845-2119, USA. b-patil@tamu.edu" |
| DOI: | 10.1007/s11306-018-1385-1 |
| ISSN/ISBN: | 1573-3890 (Electronic) 1573-3882 (Linking) |
| Abstract: | "INTRODUCTION: In recent years, growers have used various production types, including high-tunnel systems, to increase the yield of tomatoes (Lycopersicon esculentum). However, the effect of high-tunnel cultivation, in comparison to conventional open-field production, on aroma and flavor volatiles is not fully understood. OBJECTIVES: To optimize the extraction and quantification conditions for the analysis of tomato volatiles using headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS), and study the effect of production systems on volatile profiles using metabolomics approach. METHODS: The HS-SPME conditions were optimized for extraction and GC-MS was used to quantify the volatiles from four tomato varieties grown in open-field and high-tunnel systems. Univariate and multivariate analyses were performed to identify the influence of production system on tomato volatiles. RESULTS AND CONCLUSIONS: The extraction of 2 g tomato samples at 60 degrees C for 45 min using divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber gave the maximum amounts of volatiles. This optimized method was used to identify and quantify 41 volatiles from four tomato varieties. The levels of beta-damascenone were higher in the high-tunnel tomatoes and geranylacetone was higher in open-field tomatoes. These two volatile compounds could be considered as biomarkers for tomatoes grown in high-tunnel and open-field production systems. This study is the first report comparing volatiles in tomatoes grown in high-tunnel and open-field conditions, and our results confirmed that there is a critical need to adopt biomarker-specific production systems to improve the nutritional and organoleptic properties of tomatoes" |
| Keywords: | Biomarkers/analysis Gas Chromatography-Mass Spectrometry Solanum lycopersicum/*chemistry *Metabolomics *Solid Phase Microextraction Volatile Organic Compounds/*analysis Gc-ms Hs-spme High-tunnel Metabolomics Tomato Volatiles; |
| Notes: | "MedlineLee, Jisun H J Jayaprakasha, G K Rush, Charlie M Crosby, Kevin M Patil, Bhimanagouda S eng 2015-121277/Texas State Funding/International Research Support, Non-U.S. Gov't 2019/03/05 Metabolomics. 2018 Jul 11; 14(7):99. doi: 10.1007/s11306-018-1385-1" |
