| Title: | Role of phosphatidylserine in the localization of cell surface membrane proteins in yeast |
| Author(s): | Kashikuma R; Nagano M; Shimamura H; Nukaga K; Katsumata I; Toshima Y; Toshima J; |
| Address: | "Department of Biological Science and Technology, Tokyo University of Science. School of Health Science, Tokyo University of Technology" |
| ISSN/ISBN: | 1347-3700 (Electronic) 0386-7196 (Linking) |
| Abstract: | "Phosphatidylserine (PS) is a constituent of the cell membrane, being especially abundant in the cytoplasmic leaflet, and plays important roles in a number of cellular functions, including the formation of cell polarity and intracellular vesicle transport. Several studies in mammalian cells have suggested the role of PS in retrograde membrane traffic through endosomes, but in yeast, where PS is localized primarily at the plasma membrane (PM), the role in intracellular organelles remains unclear. Additionally, it is reported that polarized endocytic site formation is defective in PS-depleted yeast cells, but the role in the endocytic machinery has not been well understood. In this study, to clarify the role of PS in the endocytic pathway, we analyzed the effect of PS depletion on endocytic internalization and post-endocytic transport. We demonstrated that in cell lacking the PS synthase Cho1p (cho1Delta cell), binding and internalization of mating pheromone alpha-factor into the cell was severely impaired. Interestingly, the processes of endocytosis were mostly unaffected, but protein transport from the trans-Golgi network (TGN) to the PM was defective and localization of cell surface proteins was severely impaired in cho1Delta cells. We also showed that PS accumulated in intracellular compartments in cells lacking Rcy1p and Vps52p, both of which are implicated in endosome-to-PM transport via the TGN, and that the number of Snx4p-residing endosomes was increased in cho1Delta cells. These results suggest that PS plays a crucial role in the transport and localization of cell surface membrane proteins.Key words: phosphatidylserine, endocytosis, recycling, vesicle transport" |
| Keywords: | Cell Membrane/metabolism Endocytosis/physiology Endosomes/metabolism *Membrane Proteins/metabolism Phosphatidylserines/metabolism Protein Transport *Saccharomyces cerevisiae/metabolism trans-Golgi Network/metabolism endocytosis phosphatidylserine recyclin; |
| Notes: | "MedlineKashikuma, Ryutaro Nagano, Makoto Shimamura, Hiroki Nukaga, Kouya Katsumata, Ikumi Y Toshima, Junko Toshima, Jiro eng Japan 2022/12/15 Cell Struct Funct. 2023 Jan 28; 48(1):19-30. doi: 10.1247/csf.22081. Epub 2022 Dec 15" |
