Title: | Sheep pancreatic microsomes as an alternative to the dog source for studying protein translocation |
Author(s): | Kaderbhai MA; Harding VJ; Karim A; Austen BM; Kaderbhai NN; |
Address: | "Institute of Biological Sciences, University of Wales, Penglais, Aberystwyth, U.K" |
ISSN/ISBN: | 0264-6021 (Print) 1470-8728 (Electronic) 0264-6021 (Linking) |
Abstract: | "A procedure is described for the preparation of rough membrane vesicles of endoplasmic-reticular origin from the pancreas of sheep. These isolated membranes translocate, process and glycosylate in vitro-translated heterologous proteins in a manner comparable with that exhibited by dog pancreatic microsomes" |
Keywords: | "Animals Biological Transport *Dogs Endopeptidases/metabolism Endoplasmic Reticulum/*metabolism/ultrastructure Escherichia coli/enzymology Intracellular Membranes/metabolism/ultrastructure Mating Factor *Membrane Proteins Microscopy, Electron Microsomes/*m;" |
Notes: | "MedlineKaderbhai, M A Harding, V J Karim, A Austen, B M Kaderbhai, N N eng Comparative Study Research Support, Non-U.S. Gov't England 1995/02/15 Biochem J. 1995 Feb 15; 306 ( Pt 1)(Pt 1):57-61. doi: 10.1042/bj3060057" |