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J Biosci

Title:		"RNA silencing of hormonal biosynthetic genes impairs larval growth and development in cotton bollworm, Helicoverpa armigera"
Author(s):		Jaiwal A; Natarajaswamy K; Venkat Rajam M;
Address:		"Department of Genetics, University of Delhi - South Campus, Benito Juarez Road, New Delhi 110021, India"
Journal Title:		J Biosci
Year:		2020
Volume:		45
Issue:
Page Number:		-
DOI:
ISSN/ISBN:		0973-7138 (Electronic) 0250-5991 (Linking)
Abstract:		"The cotton bollworm, Helicoverpa armigera, is a highly polyphagous pest, causing enormous losses to various economically important crops. The identification and in vitro functional validation of target genes of a pest is a prerequisite to combat pest via host-mediated RNA interference (RNAi). In the present study, six hormonal biosynthesis genes of H. armigera were chosen and evaluated by feeding insect larvae with dsRNAs corresponding to each target gene, viz., juvenile hormone acid methyltransferase (HaJHAMT), prothoracicotropic hormone (HaPTTH), pheromone biosynthesis-activating peptide (HaPBAP), molt regulating transcription factor (HaHR3), activated protein 4 (HaAP-4) and eclosion hormone precursor (HaEHP). The loss of function phenotypes for these hormonal genes were observed by releasing second instar larvae on to artificial diet containing target gene-specific dsRNAs. Ingestion of dsRNAs resulted in mortality ranging from 60% to 90%, reduced larval weight, phenotypic deformities and delayed pupation. The quantitative real-time PCR (qRT-PCR) analysis showed that the target gene transcript levels were decreased drastically (31% to 77%) as compared to control or unrelated control (GFP-dsRNA), and correlated well with the mortality and developmental defects of larvae. Also, a comparison of the silencing efficacy of un-diced long HaPTTH -dsRNAwith RNase III diced HaPTTH-dsRNA (siRNAs) revealed that long dsRNAs were more efficient in silencing the target gene. These results indicated that the hormonal biosynthesis genes have varied sensitivity towards RNAi and could be the vital targets for insect resistance in crop plants like cotton which are infested by H. armigera"
Keywords:		"Animals Gene Expression Regulation, Developmental Genes, Reporter Gossypium/parasitology Green Fluorescent Proteins/antagonists & inhibitors/genetics/metabolism Insect Control/*methods Insect Hormones/antagonists & inhibitors/genetics/metabolism Insect Pr;"
Notes:		"MedlineJaiwal, Anjali Natarajaswamy, Kalleda Venkat Rajam, Manchikatla eng India 2020/09/26 J Biosci. 2020; 45:109"