| Title: | Sol-gel-based SPME fiber as a reliable sampling technique for studying biogenic volatile organic compounds released from Clostridium tetani |
| Author(s): | Ghader M; Shokoufi N; Es-Haghi A; Kargosha K; |
| Address: | "Faculty of Clean Technologies, Chemistry & Chemical Engineering Research Center of Iran (CCERCI), P.O. Box 14968-13151, Tehran, Iran. Department of Physico Chemistry, Razi Vaccine & Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148, Karaj, Iran. Department of Physico Chemistry, Razi Vaccine & Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148, Karaj, Iran. a.eshaghi@rvsri.ac.ir" |
| DOI: | 10.1007/s00216-017-0675-1 |
| ISSN/ISBN: | 1618-2650 (Electronic) 1618-2642 (Linking) |
| Abstract: | "A novel and efficient headspace solid-phase microextraction (HS-SPME) method, followed by gas chromatography mass spectrometry (GC-MS), was developed to study volatile organic compounds (VOCs) emerging from microorganisms. Two homemade SPME fibers, a semi-polar poly (dimethylsiloxane) (PDMS) fiber, and a polar polyethylene glycol (PEG) fiber, along with two commercial fibers (PDMS and PDMS/DVB) were used to collect VOCs emerging from Clostridium tetani which was cultured in different media. The adsorbed VOCs were desorbed and identified, in vitro, using GC-MS. The adsorption efficiency was improved by optimizing the time duration of adsorption and desorption. About 50 components were identified by the proposed method. The main detected compounds appeared to be sulfur containing compounds such as butanethioic acid S-methyl ester, dimethyl trisulfide, and dimethyl tetrasulfide. These volatile sulfur containing compounds are derived from amino acids containing the sulfur element, which probably coexist in the mentioned bacterium or are added to the culture media. The developed HS-SPME-GC-MS method allowed the determination of the chemical fingerprint of Clostridium tetani volatile constituents, and thus provides a new, simple, and reliable tool for studying the growth of microorganisms. Graphical abstract Investigation of biogenic VOCs released from Clostridium tetani using SPME-GC-MS" |
| Keywords: | Adsorption Clostridium tetani/*chemistry Dimethylpolysiloxanes/chemistry Gas Chromatography-Mass Spectrometry Polyethylene Glycols/chemistry Solid Phase Microextraction/*methods Volatile Organic Compounds/*analysis/chemistry Clostridium tetani Gc-ms Hs-sp; |
| Notes: | "MedlineGhader, Masoud Shokoufi, Nader Es-Haghi, Ali Kargosha, Kazem eng Germany 2017/10/17 Anal Bioanal Chem. 2017 Nov; 409(29):6739-6744. doi: 10.1007/s00216-017-0675-1. Epub 2017 Oct 13" |
