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J Insect Physiol

Title:		"Pheromonal activities of the bombykol isomer, (10E,12E)-10,12-hexadecadien-1-ol, in the pheromone gland of the silkmoth Bombyx mori"
Author(s):		Fujii T; Sakurai T; Mitsuno H; Matsuyama S; Shiota Y; Ito K; Yokoyama T; Nishioka T; Katsuma S; Kanzaki R; Ishikawa Y;
Address:		"Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan; Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo 183-8509, Japan. Electronic address: takeshi.f0405@gmail.com. Research Center for Advanced Science and Technology, The University of Tokyo, Meguro-ku, Tokyo 153-8904, Japan; Faculty of Agriculture, Tokyo University of Agriculture, Atsugi, Kanagawa 243-0034, Japan. Research Center for Advanced Science and Technology, The University of Tokyo, Meguro-ku, Tokyo 153-8904, Japan. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan. Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo 183-8509, Japan. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan"
Journal Title:		J Insect Physiol
Year:		2020
Volume:		20200124
Issue:
Page Number:		104018 -
DOI:		10.1016/j.jinsphys.2020.104018
ISSN/ISBN:		1879-1611 (Electronic) 0022-1910 (Linking)
Abstract:		"Bombykol (EZ) is the single component of the female sex pheromone in the silkmoth Bombyx mori. EZ alone evokes full courtship behaviors from conspecific males; however, its geometric isomer (EE) was consistently detected in the pheromone glands (PG) of 16 B. mori strains and a field population of the wild silkmoth Bombyx mandarina, which also uses EZ as the single pheromone component. We investigated the pheromonal activities of EE using a commercial hybrid strain of B. mori, Kinshu x Showa. The behavioral assay demonstrated that a 10(4)-10(5)-fold higher dose of EE than EZ was able to elicit behavioral responses from males. To elucidate whether the trace contaminant of EZ in the EE standard is responsible for these responses, we examined the responses of male antennae to EE using a gas chromatograph-electroantennographic detector system (GC-EAD). The EE, at high doses elicited marginal responses from the male antennae. We next examined antennal and behavioral responses of B. mori whose BmOR1 gene, which is responsible for the reception of bombykol, was knocked out. The knockout of BmOR1 resulted in the complete loss of antennal and behavioral responses to EE and EZ, demonstrating that if EE itself is active, it induces these responses via the incidental stimulation of BmOR1, not via the stimulation of EE-specific receptors. The existence of EE in the PG of B. mori and B. mandarina is discussed from the viewpoints of pheromone biosynthesis and the evolution of pheromone communication systems"
Keywords:		"Animals Arthropod Antennae/metabolism/*physiology *Bombyx/genetics/metabolism/physiology Chromatography, Gas *Fatty Alcohols/chemical synthesis/metabolism Gene Knockout Techniques Genes, Insect Male Olfactory Perception/genetics Pheromones/biosynthesis/ch;"
Notes:		"MedlineFujii, Takeshi Sakurai, Takeshi Mitsuno, Hidefumi Matsuyama, Shigeru Shiota, Yusuke Ito, Katsuhiko Yokoyama, Takeshi Nishioka, Takaaki Katsuma, Susumu Kanzaki, Ryohei Ishikawa, Yukio eng Research Support, Non-U.S. Gov't England 2020/01/29 J Insect Physiol. 2020 Feb-Mar; 121:104018. doi: 10.1016/j.jinsphys.2020.104018. Epub 2020 Jan 24"