| Title: | "Simplex-centroid design as innovative approach in the optimization of antimicrobial effect of Thymus satureioides, Myrtus communis and Artemisia herba alba essential oils against Escherichia coli, Staphylococcus aureus and Candidatropicalis" |
| Author(s): | Chraibi M; Fadil M; Farah A; Benkhaira N; Lebrazi S; Fikri-Benbrahim K; |
| Address: | "Laboratory of Microbial Biotechnology and Bioactive Molecules, Faculty of Sciences and Techniques Sidi Mohamed Ben Abdellah University, Fez, Morocco. Physico-Chemical Laboratory of Inorganic and Organic Materials, Materials Science Center (MSC), Ecole Normale Superieure, Mohammed V University in Rabat, Rabat, Morocco. Laboratory of Applied Organic Chemistry, Faculty of Sciences and Techniques Sidi Mohamed Ben Abdellah University, Fez, Morocco. Laboratory of Microbial Biotechnology and Bioactive Molecules, Faculty of Sciences and Techniques Sidi Mohamed Ben Abdellah University, Fez, Morocco. Electronic address: kawtar.fikribenbrahim@usmba.ac.ma" |
| DOI: | 10.1016/j.exppara.2023.108472 |
| ISSN/ISBN: | 1090-2449 (Electronic) 0014-4894 (Linking) |
| Abstract: | "Essential Oils (EOs) known since Antiquity, and initially obtained by maceration of aromatic plants, are used as diseases' remedies because they contain valuable therapeutic components. Antimicrobial effect's evaluation of formulations established by an augmented centered mixing plan for three Moroccan medicinal and aromatic plants' essential oils (EOs): Thymus satureioides (T. satureioides), Myrtus communis (M. communis) and Artemisia herba alba (A. herba alba) against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Candida tropicalis (C. tropicalis). The identification of E.Os chemical compounds was made using Gaz chromatography (GC) and Gaz chromatography coupled to Mass spectrometry (GC/MS).Whereas, the optimization of their antimicrobial effect was performed by experimental design methodology (mixture design) coupled to microdilution method based on Minimal Inhibitory Concentrations (MICs) determination. Results showed that the main components are borneol (34.26%) and alpha-pinene (31.21%); borneol (27.15%) and 1,8-cineole (21.33%); camphor (14.67%), chrysanthenone (14.10%), and beta-thujone (12.60%) for T. satureioides, M. communis and A. herba alba respectively. Optimal mixtures composed of 60% and 40% of T. satureioides and M. communis E.Os; 72% and 28% of T. satureioides and A. Herba alba E.Os; 75% of thyme E.O and 25% of mugwort E.O; predicted the highest antimicrobial effect against E. coli, S. aureus and C. tropicalis, respectively. This study highlights the antimicrobial effect of EOs tertiary mixtures through using mixture design methodology, which may contribute to a successful application in pharmaceutical, food, or cosmetic industries" |
| Keywords: | "*Oils, Volatile/pharmacology/chemistry Staphylococcus aureus Escherichia coli *Myrtus/chemistry *Artemisia/chemistry *Thymus Plant *Anti-Infective Agents Antimicrobial activity Mixture design Optimization Synergy;" |
| Notes: | "MedlineChraibi, Marwa Fadil, Mouhcine Farah, Abdellah Benkhaira, Nesrine Lebrazi, Sara Fikri-Benbrahim, Kawtar eng 2023/01/25 Exp Parasitol. 2023 Apr; 247:108472. doi: 10.1016/j.exppara.2023.108472. Epub 2023 Jan 21" |
