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J Cell Biol

Title:		"Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in yeast late Golgi function"
Author(s):		Chen CY; Ingram MF; Rosal PH; Graham TR;
Address:		"Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235, USA"
Journal Title:		J Cell Biol
Year:		1999
Volume:		147
Issue:		6
Page Number:		1223 - 1236
DOI:		10.1083/jcb.147.6.1223
ISSN/ISBN:		0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking)
Abstract:		"ADP-ribosylation factor appears to regulate the budding of both COPI and clathrin-coated transport vesicles from Golgi membranes. An arf1Delta synthetic lethal screen identified SWA3/DRS2, which encodes an integral membrane P-type ATPase and potential aminophospholipid translocase (or flippase). The drs2 null allele is also synthetically lethal with clathrin heavy chain (chc1) temperature-sensitive alleles, but not with mutations in COPI subunits or other SEC genes tested. Consistent with these genetic analyses, we found that the drs2Delta mutant exhibits late Golgi defects that may result from a loss of clathrin function at this compartment. These include a defect in the Kex2-dependent processing of pro-alpha-factor and the accumulation of abnormal Golgi cisternae. Moreover, we observed a marked reduction in clathrin-coated vesicles that can be isolated from the drs2Delta cells. Subcellular fractionation and immunofluorescence analysis indicate that Drs2p localizes to late Golgi membranes containing Kex2p. These observations indicate a novel role for a P-type ATPase in late Golgi function and suggest a possible link between membrane asymmetry and clathrin function at the Golgi complex"
Keywords:		ADP-Ribosylation Factor 1/genetics/physiology Amino Acid Sequence Aspartic Acid/genetics/metabolism Biological Transport Calcium-Transporting ATPases/chemistry/genetics/*metabolism Carrier Proteins/chemistry/genetics/*metabolism Clathrin/genetics/physiolo;
Notes:		"MedlineChen, C Y Ingram, M F Rosal, P H Graham, T R eng Research Support, U.S. Gov't, Non-P.H.S. 1999/12/22 J Cell Biol. 1999 Dec 13; 147(6):1223-36. doi: 10.1083/jcb.147.6.1223"