| Title: | Volatile metabolites differentiate air-liquid interface cultures after infection with Staphylococcus aureus |
| Author(s): | Ahmed W; Bardin E; Davis MD; Sermet-Gaudelus I; Grassin Delyle S; Fowler SJ; |
| Address: | "Division of Immunology, Immunity to infection & Respiratory Medicine, Faculty of Biology, Medicine, and Health, University of Manchester, Manchester, UK. Institut Necker-Enfants Malades, Paris, France. Universite Paris-Saclay, UVSQ, INSERM, Infection et inflammation, Montigny le Bretonneux, France. Herman B Wells Center for Pediatric Research, Pediatric Pulmonology, Allergy, and Sleep Medicine, Indiana University School of Medicine, Indianapolis, USA. Service de Pneumo-Pediatrie, Universite Rene Descartes, Hopital Necker-Enfants Malades, Paris, France. Hopital Foch, Exhalomics, Departement des maladies des voies respiratoires, Suresnes, France. NIHR Manchester Biomedical Research Centre, Manchester University Hospitals NHS Foundation Trust, Education and Research Centre, W ythenshawe Hospital, Manchester, M23 9LT, UK. stephen.fowler@manchester.ac.uk" |
| ISSN/ISBN: | 1364-5528 (Electronic) 0003-2654 (Linking) |
| Abstract: | "Early detection of lung infection is critical to clinical diagnosis, treatment, and monitoring. Measuring volatile organic compounds (VOCs) in exhaled breath has shown promise as a rapid and accurate method of evaluating disease metabolism and phenotype. However, further investigations of the role and function of VOCs in bacterial-host-stress response is required and this can only be realised through representative in vitro models. In this study we sampled VOCs from the headspace of A549 cells at an air-liquid interface (ALI). We hypothesised VOC sampling from ALI cultures could be used to profile potential biomarkers of S. aureus lung infection. VOCs were collected using thin film microextraction (TFME) and were analysed by thermal desorption-gas chromatography-mass spectrometry. After optimising ALI cultures, we observed seven VOCs changed between A549 and media control samples. After infecting cells with S. aureus, supervised principal component-discriminant function analysis revealed 22 VOCs were found to be significantly changed in infected cells compared to uninfected cells (p < 0.05), five of which were also found in parallel axenic S. aureus cultures. We have demonstrated VOCs that could be used to identify S. aureus in ALI cultures, supporting further investigation of VOC analysis as a highly sensitive and specific test for S. aureus lung infection" |
| Keywords: | *Staphylococcus aureus/metabolism *Volatile Organic Compounds/analysis Bacteria/metabolism Discriminant Analysis Biomarkers/analysis Breath Tests/methods; |
| Notes: | "MedlineAhmed, Waqar Bardin, Emmanuelle Davis, Michael D Sermet-Gaudelus, Isabelle Grassin Delyle, Stanislas Fowler, Stephen J eng England 2023/01/05 Analyst. 2023 Jan 31; 148(3):618-627. doi: 10.1039/d2an01205g" |
